GGT to GAT mutation in base 2 of codon 12.
GGT to GAT mutation in base 2 of codon 12.
Normal genotype in codons 12 and 13.
Normal genotype in codons 12 and 13.
GGT to AGT mutation in base 1 of codon 12.
GGT to AGT mutation in base 1 of codon 12.
Reanalysis of the data in figure
Reanalysis of the data in figure "GGT to AGT mutation in base 1 of codon 12".
Principle of Pyrosequencing — step 1.
Principle of Pyrosequencing — step 1.
<p>Principle of Pyrosequencing — step 2.</p>

Principle of Pyrosequencing — step 2.
<p>Principle of Pyrosequencing — step 3.</p>

Principle of Pyrosequencing — step 3.
Principle of Pyrosequencing — step 4.
Principle of Pyrosequencing — step 4.
Principle of Pyrosequencing — step 5.
Principle of Pyrosequencing — step 5.
Workflow solutions.
Workflow solutions.
Fully integrated system.
Fully integrated system.
Easy data management.
Easy data management.
Efficient template prep.
Efficient template prep.
The right instrument for your needs.
The right instrument for your needs.
GGT to GAT mutation in base 2 of codon 12. Nucleotide 35 is indicated with an arrow.
Normal genotype in codons 12 and 13.
GGT to AGT mutation in base 1 of codon 12. The Pyrogram shows the mutation (nucleotide 34, indicated with an arrow) with the "Sequence to Analyze" GNTGRCGTAGGYA targeting base 2 in codon 12 (nucleotide 35). The yellow color indicates that this sequence is unexpected and needs to be checked.
Reanalysis of the data in figure The mutation GGT → AGT was reanalyzed with the "Sequence to Analyze" NGTGRCGTAGGYA targeting base 1 in codon 12 (nucleotide 34). With this new targeted sequence to analyze, the software rates the assay as one of good quality, confirming the quantification of the mutation.
Principle of Pyrosequencing — step 1.
<p>Principle of Pyrosequencing — step 2.</p>
<p>Principle of Pyrosequencing — step 3.</p>
Principle of Pyrosequencing — step 4.
Principle of Pyrosequencing — step 5.
Workflow solutions.

The components of the PyroMark Q24 MDx System are designed to make the Pyrosequencing workflow straightforward and efficient. Each step, from assay design to PCR amplification and preparation of sequencing templates, is supported by software, kits, reagents, and sample prep instrumentation optimized for Pyrosequencing.
Fully integrated system.

Though small in size, the PyroMark Q24 MDx manages all steps necessary to rapidly analyze up to 24 samples. Simply load samples and reagents, upload the run file, and walk away. The PyroMark Q24 MDx dispenses reagents and nucleotides to each well with precision. Light signals emitted are detected by 24 CCD sensors — one sensor per well — thereby eliminating signal crossover.
Easy data management.

The PyroMark Q24 MDx is designed as a stand-alone instrument, which makes it easy to place anywhere in the lab. Data are stored on the instrument hard drive and can be viewed on the instrument screen during a run. Additionally, all files are stored on the supplied USB stick, giving the user the flexibility to analyze data on any computer with PyroMark Q24 MDx Software installed.
Efficient template prep.

The PyroMark Q24 MDx Vacuum Workstation enables conversion of PCR products into the single-stranded DNA needed as template for Pyrosequencing. Exposure of the PCR amplicons to a series of optimized solutions denatures and washes the DNA. This process is carried out for 24 samples in parallel and takes only a few minutes.
The right instrument for your needs.

The PyroMark Q24 MDx combines ease of use, analysis versatility, and superior detection sensitivity to meet the needs of your diagnostic work. For research purposes, we also offer the PyroMark Q96 ID and PyroMark Q96 MD that can process up to 96 samples in a single run. The PyroMark Q96 MD can additionally be automated to run ten 96-well plates.