For the isolation of total RNA from stool and gut material
  • Fast and easy protocol isolates high-quality total RNA in less than 45 minutes
  • Efficient isolation of total RNA from difficult microbiome samples
  • Highly pure RNA isolation with Inhibitor Removal Technology
  • Simple co-isolation of miRNA and DNA using an on-column procedure

Get high-quality RNA fast, free of lingering PCR inhibitors with the RNeasy PowerMicrobiome Kit. Designed for purification of total RNA from stool, dried feces, gut material, contaminated buccal swabs and secretions, the RNeasy Power Microbiome Kit isolates RNA that is ready for demanding downstream applications including RT-PCR, qPCR and RNA-seq. Digested food, heme from lysed red blood cells in stool and other PCR inhibitors are no match for the Inhibitor Removal Technology that is included in the kit. The RNeasy PowerMicrobiome Kit also isolates miRNA and can optionally co-isolate DNA using a simple, on-column procedure using reagents provided with the kit.

Isolation of RNA using the RNeasy PowerMicrobiome Kit can be automated on the QIAcube Connect.

RNeasy PowerMicrobiome Kit was formerly sold by MO BIO as PowerMicrobiome RNA Isolation Kit.

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RNeasy PowerMicrobiome Kit (50)
For the isolation of total RNA from stool and gut material
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IRS Solution (11 ml)
RNeasy PowerMicrobiome Kit Components
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Solution PM1 (55 ml)
RNeasy PowerMicrobiome Kit Components
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Solution PM3 (36 ml)
RNeasy PowerMicrobiome Kit Components
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Solution PM4 (72 ml)
RNeasy PowerMicrobiome Kit Components
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Solution PM5 (90 ml)
RNeasy PowerMicrobiome Kit Components
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The RNeasy PowerMicrobiome Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Figure 1. High-quality, intact total RNA isolated from human stool using the RNeasy PowerMicrobiome Kit.|Figure 2. RNeasy PowerMicrobiome Kit isolated high-quality total RNA from canine stool samples.|
Healthy volunteer samples were collected and total RNA purified using the RNeasy PowerMicrobiome Kit. Profiling and quantification of 1 µl of RNA was performed using an Agilent Bioanalyzer RNA Nano Kit chip. In the electropherogram, clear 16S and 23S bands are shown for prokaryotic rRNA. RNA processed through the RNeasy PowerMicrobiome Kit was demonstrated to be high-quality by an RIN of 9.4. (Data kindly provided by C. Vincent, McGill University, Montreal, Canada.)|Experimental RNA preservation solution was used to preserve fresh stool samples at room temperature or on ice. Collection and processing occurred within the same 24-hour period. RNeasy PowerMicrobiome Kit was used to isolate total RNA. Each sample (10 µl per) was examined on a 1% agarose gel.|

Format Silica Spin Filter Tubes
Sample size 0.25 g
Throughput 1-24 samples
Time per run or per prep 45 minutes
Storage temperature Store lyophilized DNase I at 4°C
All other kit reagents and components should be stored at room temperature (15-30°C)
Bead size 0.1 mm glass
Sample types Stool, gut material, dried feces, contaminated buccal swabs and secretions

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This webinar focuses on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome.