For the isolation of high-quality, pure DNA from biofilm samples

  • High DNA yields from difficult biofilm samples
  • Inhibitor Removal Technology provides high-quality DNA for downstream applications
  • Optimized isolation of pure DNA from all biofilms, including dental plaques and microbial mats

By pairing chemical and mechanical lysis techniques and Inhibitor Removal Technology (IRT), the DNeasy PowerBiofilm Kit overcomes inefficient cell lysis in the most challenging biofilms. IRT removes concentrated inhibitors such as humic acid, metals, salts and pesticides, typically present in biofilms resulting in increased yields of inhibitor-free DNA from all types of biofilms, including microbial mats.

Purification of DNA using the DNeasy PowerBiofilm Kit can be automated on the QIAcube Connect.

DNeasy PowerBiofilm Kit was formerly sold by MO BIO as PowerBiofilm DNA Isolation Kit.

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Product Cat. no. List price:
DNeasy PowerBiofilm Kit (50)
For the isolation of high-quality, pure DNA from biofilm samples
24000-50
$431.00
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The DNeasy PowerBiofilm Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Figure 1: High-quality biofilm DNA isolated from a range of locations.|Figure 2: Pure DNA from stream biofilm.|Figure 3: PCR-ready DNA from any biofilm.|
Biofilm DNA was isolated from a variety of locations. NanoDrop provided DNA yield. Agarose gel (1.2%) was used to assess quality. Each biofilm sample provided high-quality, high yield DNA when processed with the DNeasy PowerBiofilm Kit.|The DNeasy PowerBiofilm Kit was used to extract DNA from stream epilithic biofilms. Biofilm was removed from stream pebbles using sonication. The sample was then pelleted through centrifugation, resuspended in Solution MBL and transferred to the bead tube. (Data kindly provided by A.T. Gildemeister, University of Stirling, UK)|PCR performed using the KAPA 2GTM Fast HotStart Kit. Universal 16S rRNA gene (StreF/StreR) primers were used. This generated a 1,243 bp product.|
Performance


Feature
Specifications
Format Silica Spin Filter Tubes
Processing Bead Beating
Sample size 0.05 - 0.20 g
Binding capacity Up to 20 µg per prep
Throughput 1-24 samples
Time per run or per prep 25 minutes
Storage temperature Store at room temperature (15-30°C)
Sample types All processed biofilms from cultured single cell layers to microbial mats
Bead size 0.1 mm glass, 0.5 mm glass, 2.4 mm ceramic mix

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Webinars
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This webinar focuses on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome.