Mechanism of fast cycling during annealing.
Mechanism of fast cycling during annealing.
Unique multiplex PCR buffer promotes stable and efficient annealing.
Unique multiplex PCR buffer promotes stable and efficient annealing.
Principle of the novel QuantiNova two-phase hot-start mechanism.
Principle of the novel QuantiNova two-phase hot-start mechanism.
Accurate reaction setup indicated by the built-in pipetting control.
Accurate reaction setup indicated by the built-in pipetting control.
Mechanism of fast cycling during annealing. [A] The proprietary PCR buffer contains Q-Bond, a molecule that facilitates the reduction of the annealing step to 5 seconds. Q-Bond increases the affinity of Taq DNA polymerases for short single-stranded DNA fragments, reducing the time needed for successful primer annealing. [B] Without Q-Bond, the primer and polymerase bind sequentially to the template, increasing the duration of the annealing step.
Unique multiplex PCR buffer promotes stable and efficient annealing. [A] NH4+ ions prevent nonspecific primers from annealing to the template. Synthetic Factor MP, an innovative PCR additive, increases the local concentration of primers at the template. [B] Together with K+ and other cations, synthetic Factor MP stabilizes specifically bound primers, allowing efficient primer extension by DNA Polymerase.
Principle of the novel QuantiNova two-phase hot-start mechanism. At ambient temperature the HotStaRTScript is inhibited by the RT-Blocker and the QuantiNova DNA Polymerase is kept inactive by QuantiNova Antibody and QuantiNova Guard. At 50°C the RT is activated while the QuantiNova DNA polymerase remains inactive. At 95°C the RT enzyme is denatured and the DNA polymerase is activated.
Accurate reaction setup indicated by the built-in pipetting control.

The master mix contains an inert blue dye. Combined with QuantiNova Yellow Template Dilution Buffer, the resulting solution turns green, indicating that the reaction was set up correctly.