Quantifications of gDNA on the QIAxpert are in high concordance with actual concentrations.
Comprehensive reports of measurements are available in several formats for data analysis and sharing.
Expert design of the QIAxpert Slide ensures clean sample loading, flexible use of sample positions, and preservation of samples for up to 2 hours.
[A] A highly pure gDNA sample shows no significant contamination as evidenced by the spectrum. Only the blue spectrum for DNA is present, while those for impurities (orange), background (gray) and residue (yellow) are not. [B] An RNA sample was spiked with gDNA. The RNA is indicated by the blue absorbance line, and the spiked DNA is represented by the orange absorbance line. [C] An RNA sample was spiked with phenol. The RNA is indicated by the blue absorbance line, and the spiked phenol is represented by the orange absorbance line. [D] A gDNA sample was spiked with hemoglobin. The gDNA is indicated by the blue absorbance line, and the spiked hemoglobin is represented by the gray absorbance line. The black absorbance curve indicates the total measured, background-corrected spectrum of the sample; the sum of DNA and RNA is shown as a pink absorbance curve (shown only if no differentiation between DNA and RNA can be performed); the blue absorbance line indicates the target molecule (DNA or RNA; app-dependent); the orange spectrum indicates impurities (DNA or RNA, salts, phenol; app-dependent); the gray absorbance curve shows the measured background of the sample (turbidity, beads, hemoglobin, chlorophyll; app-dependent); the yellow line indicates the unknown part of a sample that cannot be assigned to the above mentioned spectral parts.
