QIAxcel Advanced System

For effortless DNA and RNA analysis

Features

  • Rapid analysis of up to 96 samples without manual intervention
  • Safety and convenience with ready-to-use gel cartridges
  • Accurate analysis of low concentration nucleic acids with 3–5 bp resolution
  • User-friendly analysis software that supports electronic signatures and records
  • Objective RNA quality measurement with new RNA Integrity Score (RIS)
QIAxcel Advanced Instrument

Cat. No. / ID: 9001941

Capillary electrophoresis device: includes computer, QIAxcel ScreenGel Software, and 1-year warranty on parts and labor
InstrumentSoftware
QIAxcel Advanced
QIAxcel ScreenGel Software
Service Options
Warranty
System
Priority
PrioPLUS
The QIAxcel Advanced instrument is intended to be used only in combination with QIAxcel Kits for the applications described in the respective QIAxcel Kit handbooks.

Product Details


The revolutionary QIAxcel Advanced system replaces traditional, labor-intensive gel analysis of DNA and RNA — streamlining workflows and reducing time to result. The QIAxcel Advanced system fully automates sensitive, high-resolution capillary electrophoresis of up to 96 samples per run. DNA fragment analysis of 12 samples can be performed in as little as 3 minutes. Ready-to-run gel cartridges allow 96 samples to be analyzed with a minimum of hands-on interaction, reducing manual handling errors and eliminating the need for tedious gel preparation. User-friendly QIAxcel ScreenGel software ensures convenient analysis and documentation of data. The QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement of the analyzed samples and allows easy interpretation of sample integrity.

Explore the virtual demo and watch a video to learn more about the QIAxcel Advanced system.

The QIAxcel Advanced system operates in conjunction with the QIAxcel DNA Kits and QIAxcel RNA QC Kit v2.0.

Performance

Streamlined workflow
The QIAxcel Advanced system not only speeds up DNA and RNA analysis by eliminating slab-gel analysis, but also contributes to streamlining the entire sample purification and analysis workflow. Used in combination with proven QIAGEN end-point PCR kits, the QIAxcel Advanced provides an all-in-one, pretested solution for reliable analysis of PCR fragments, ensuring reproducibility and significant time and cost savings.

One instrument, many applications
The QIAxcel Advanced system is a versatile solution for electrophoresis and offers a broad range of applications. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids — including single or multiple PCR fragments, DNA digested with restriction endonucleases, total RNA, and cRNA. Unlike other commercially available devices that provide processing of only up to 12 samples at time, the QIAxcel Advanced system allows processing of all 96 samples without manual intervention.

DNA analysis
In genotyping applications, the QIAxcel Advanced system enables greater sizing accuracy and more sensitive detection than conventional agarose gel electrophoresis (see figure  Accurate, high-throughput genotyping of bacteria). QIAxcel Advanced also provides a straightforward and effective means for checking the quality of genomic DNA (gDNA) samples prior to molecular screening and downstream assays (see figure  Assessment of DNA degraded by sonication).

RNA analysis — objective quality values with the RNA Integrity Score
The new QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement for eukaryotic RNA samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure  RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.

Such careful monitoring of the initial RNA sample, as well as products generated throughout the entire procedure, is crucial in microarray analysis, since RNA degradation strongly influences the predictive power obtained from the microarray data. The QIAxcel Advanced System is highly suitable for analyzing the integrity of total RNA and size distribution of fragmented or intact cRNA (see figure  Streamlined RNA analysis using the QIAxcel system), and the throughput capacity makes it very convenient for laboratories employing 96-well RNA purification technologies that need a fast solution for quality control.

See figures

Principle

The QIAxcel Advanced instrument includes an array of light-emitting diodes and micro-optical collectors that latch to capillaries within QIAxcel gel cartridges. Fragments that are migrating through a gel matrix within the capillary pass excitation and detection spots and the signal is transmitted through a photomultiplier tube to the QIAxcel ScreenGel software for data interpretation (see figure  Sample separation process).

The high detection sensitivity provided by the QIAxcel Advanced instrument enables robust results even with low concentrations of nucleic acid. With a resolution of 3–5 bp for fragments smaller than 0.5 kb, the QIAxcel Advanced system ensures greater accuracy than slab-gel methods, as well as greater confidence in data interpretation. Sample consumption is less than 0.1 μl per analysis, saving precious samples for further downstream analysis. Hands-free sample loading and self-contained components minimize exposure to hazardous chemicals, such as ethidium bromide (see figure  Ready-to-run gel cartridges).

A range of QIAxcel Kits is available for a variety of applications, meeting different resolution and speed requirements (see table below).

QIAxcel Kits
QIAxcel Kit Analyte Size range 100 bp – 500 bp* 500 bp – 1 kb* 1 kb – 5 kb* 5 kb – 10 kb* Run time/
12 samples
QIAxcel DNA High Resolution Kit DNA 15 bp–10 kb 3–5 bp 50 bp 200–500 bp 1–1.5 kb 7–20 min
QIAxcel DNA Screening Kit DNA 15 bp–5 kb 20–50 bp 50–100 bp 500 bp 5 min
QIAxcel DNA Fast Analysis Kit DNA 15 bp–3 kb 50–100 bp 100–250 bp 250 bp – 1 kb 3–5 min
QIAxcel RNA QC Kit v2.0 RNA 15 b–6 kb 10 min
* Best resolution. Run time depends on method used. Best resolution between 1–3 kb.
See figures

Procedure

Just a few, simple steps are required to operate the QIAxcel Advanced system for analysis of DNA and RNA samples: load the gel cartridge, fill and load the buffer tray, load the samples in 96-well plates or in PCR tubes or strips, select the process profile to be used — and go! Tedious gel preparation and extensive user training are eliminated, streamlining the workflow and facilitating integration of the system into the daily lab routine. Within minutes of starting a run, the first results appear in real time on the computer screen (see figure  Reduced run times).
See figures

Applications

The QIAxcel Advanced System offers a broad range of optimized applications for DNA and RNA analysis and is highly suited for research in academic as well as pharmaceutical, biotechnology, and biomedical laboratories. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids — including single or multiple PCR fragments, DNA digested with restriction endonucleases, synthesized oligonucleotides, total RNA, and cRNA.

The QIAxcel Advanced System has been used for applications such as:

  • Bacterial genotyping
  • PCR fragment analysis
  • Pre-sequencing testing
  • Mutation detection
  • Detection of food allergens
  • Quality control of genomic DNA
  • Total RNA and cRNA QC for RT-PCR and microarray analysis

Software

QIAxcel ScreenGel software provides a new level of convenience
QIAxcel ScreenGel software, specifically developed for use with the QIAxcel Advanced system, is a powerful and user-friendly tool for data collection and analysis. Interactive tools simplify analysis, facilitate rapid data interpretation, and provide flexibility with data and results displayed in both electropherogram and gel image formats. Results can be viewed individually or displayed as overlay views for sample and data comparison. All-in-one analysis for multiple data sets simplifies evaluation. A unique algorithm calculates and generates a tabular display of a variety of peak properties, including number of peaks as well as the size, height, width, and area of each peak. Comprehensive data reports can be easily generated and saved or exported to meet individual documentation needs. Complete process profiles for standardized sample processing — from running samples to data analysis, generating reports and exporting data — minimize the need for additional training for users (see figure  Simplified and standardized data collection and analysis).

Easy setup and start
The Process Wizard simplifies experiment startup (see figure  Easy-to-use software features). This convenient feature enables guided setup and allows run parameters to be defined and DNA size markers to be selected. Reagent lot number information can be included. Samples can be selected with a single click of the mouse and run checks can also be easily performed.

New RNA Integrity Score
The updated QIAxcel ScreenGel software now features the QIAGEN RNA Integrity Score (RIS), which enables objective quality measurement of analyzed samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure  RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.

Support of 21 CFR Part 11 compliance
QIAxcel ScreenGel software includes specific features that support the technical requirements of 21 CFR Part 11 regulations. Security features such as the following are available:
  • Password-protected login to prevent unauthorized access and data manipulation
  • Audit trail documentation for configuration files and system events
  • Automatic saving and archiving of write-protected raw data
  • Secure user management

Different user profiles are available (Routine, Basic, Advanced, Admin) and are password protected (user login is required) for increased security. A simplified interface minimizes the need for user training and makes the software especially attractive for inexperienced users.
See figures

Services

Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?

If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.

Learn more about trade-in/trade-up opportunities.

Supporting data and figures

Service Plans

QIAxcel Advanced System PRV-2

Cat. No. / ID: 9002874

Instrument and service agreement package: workstation for automated capillary electrophoresis, notebook computer and QIAxcel software; includes installation, application training and two inspection service visits. One-year warranty on labor and parts are also included. Instrument is serviced at our Regional Repair Center.
QIAxcel Advanced, PrioPLUS, no PM

Cat. No. / ID: 9002200

Instrument and service agreement package: workstation for automated capillary electrophoresis, notebook computer and QIAxcel software; includes installation, application training and Full Agreement for three years. Instrument repaired in Regional Repair Center with cost of labor, shipping and parts covered. Does not include preventive maintenance.
QIAxcel Advanced, Core Agreement

Cat. No. / ID: 9245252

One-time QIAxcel Advanced instrument repair performed at a regional repair center including shipping, labor, and spare parts for a period of one year. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service and a 10% discount on additional repair service during the Core Agreement period.
QIAxcel, Full Agreement

Cat. No. / ID: 9242699

Instrument repair service for the QIAxcel at regional repair center. Shipping, labor and parts included during the Full Agreement period. Loaner instrument provided within 2–3 business days. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service during the Full Agreement period.
QIAxcel, Preventive Subscription

Cat. No. / ID: 9241224A

One on-site Preventive Maintenance or Inspection Service visit for the QIAxcel, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

Resources

Kit Handbooks (7)
For calibration of signal intensity
For optimal DNA size determination
For optimal RNA fragment size determination
For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments
For optimal use of the QIAxcel Gel Cartridges
Operating Software (4)
This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.
This software may only be downloaded by registered users with a valid QIAxcel ScreenGel Software license. If you do not have a valid software license, contact your QIAGEN sales representative.
SHA1 checksum: 7F42EB2C97710EF11362399AC17CB7FF6899EEA1
Brochures & Guides (9)
A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher
For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader platform using ScreenGel Software version 1.5 or higher
For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader instrument
A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher
Addressing critical factors and new solutions

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

Additional Resources (8)

Method file for use with QIAxcel ScreenGel Software
 
 

Alignment marker file QX15/600 bp - English (XAM)
Method file for use with BioCalculator Software
Method file for use with BioCalculator Software
Method file for use with QIAxcel ScreenGel Software
Method file for use with QIAxcel ScreenGel Software
Process Profile and Method file for use with QIAxcel ScreenGel Software
Method file for use with BioCalculator Software
Application Notes (30)
The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.
Instrument User Manuals (1)
For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6
Instrument Technical Documents (2)

For easy upgrade of QIAxcel ScreenGel Software Version 1.6.

QIAxcel® Advanced System and 21 CFR Part 11 Regulations
White Papers (1)
This study demonstrates that the QIAxcel Advanced capillary electrophoresis system in combination with the QIAxpert UV/Vis spectrophotometer can cover the comprehensive assessment of key quality parameters of nucleic acid samples.

FAQ

If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?

Yes, if a different method is used with the QIAxcel System or QIAxcel Advanced, a new reference marker table needs to be created because of different relative migration time.

Please follow the instructions described in the manual and run the DNA marker and the Calibration marker with the new method.

FAQ ID -1857
When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?

When running RNA samples on the QIAxcel System or QIAxcel Advanced, the standard RNA denaturation process has to be followed prior to running the sample.

  • Add an equal volume of QX RNA Denaturation buffer to your RNA sample and/or QX RNA size Marker.
  • Heat the solution at 70°C for 2 minutes on a heating block or in a PCR machine, then place on ice for 1 minute.
  • Bring the total sample volume to 10 μl using QX RNA Dilution Buffer and mix the solution by gently pipetting up and down a few times.
  • Analyze the samples immediately.
FAQ ID -1842
What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced?

Make sure the Gel Cartridge has been calibrated with the computer currently connected.  If not, please re-calibrate the Gel Cartridge.

Make sure the cartridge has equilibrated to room temperature (20°–25°C [68°– 77°F]) and stood upright in the cartridge stand for 20 min prior to use. Empty the buffer tray.

Wash the buffer tray in warm water and rinse thoroughly with deionized or reverse-osmosis water. Refill the buffer tray. Perform a control run with the alignment marker and bland samples (10 μl QX DNA Dilution Buffer). The marker bands should now appear within the chosen separation time.

FAQ ID -1833
How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?

 

Refer to Appendix D of the QIAxcel DNA Handbook for hot water soaking and gel droplet testing.  The handbook can be accessed by going to the Resources tab in the link below:

http://www.qiagen.com/products/catalog/automated-solutions/detection-and-analysis/qiaxcel-dna-kits#

 

 

FAQ ID -1838
Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?

The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.

FAQ ID -1824
Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?

Unplug the QIAxcel or QIAxcel Advanced instrument and remove the fuse holder to verify that the fuses are good. If not, please replace the fuses (Cat No. 9241178 Fuse, 4A, 250VAC, QX). If this does not correct the problem, contact QIAGEN Technical Services.

FAQ ID -1851
What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?

If lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly, open the channel of the sample that was incorrectly aligned. Check whether any unexpected extra peaks have been detected or if the alignment marker peaks do have any “shoulders” that are detected as separate peaks. Delete any extra peaks if necessary and re-analyze your data.

FAQ ID -1834
If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?

The Gel Cartridge does not need to be recalibrated when using a new alignment marker and/or DNA size marker with the QIAxcel System or QIAxcel Advanced. Calibration is required only once, i.e. when the first cartridge is run or when the cartridge is used on a different QIAxcel system than the one it has been calibrated on, or when using a different PC than the one used to calibrate initially.

FAQ ID -1858
What is the QIAxcel Advanced System?

The revolutionary QIAxcel Advanced System replaces traditional, labor-intensive gel analysis of DNA and RNA.

  • Rapid analysis of up to 96 samples without manual intervention
  • Safety and convenience with ready-to-use gel cartridges
  • Robust results for nucleic acid concentrations as low as 0.1 ng/ul
  • Standardized and accurate analysis with a resolution down to 3-5 bp
  • User-friendly analysis software that supports 21 CFR Part 11 compliance

 

See:  http://www.qiagen.com/Knowledge-and-Support/Videos-and-Virtual-Demos/Assay-Demos/QIAxcelAdvancedDemo/  for a virtual demonstration of the special software, features, and applications of the QIAxcel Advanced System.

 

FAQ ID -2650
Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?

The working alignment marker is an indication that the cartridge channel is working well. DNA samples that do not appear in all channels of the QIAxcel System or QIAxcel Advanced may be caused by:

  1. Low sample volume (< 10 ul). The minimum required sample volume is 10 ul. 
  2. Trapped air bubble in the well. The air bubble must be removed. 
  3. High salt concentration in the sample. Dilute the sample with the QX DNA or RNA dilution buffer and rerun the sample.
FAQ ID -1844
Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??

Verify that the Cartridge Purge Port label is removed. If it has been removed, verify that the alignment marker and samples are in the appropriate locations in the Buffer Tray and the 96-well Sample Tray used with the QIAxcel System or QIAxcel Advanced?.

FAQ ID -1847
Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?

When using this specific marker combination with the QIAxcel System or QIAxcel Advanced, i.e. for STR analysis, the 1.8 kb fragment lies outside of the range of detectable fragments and does not show up on the electropherogram and/or the gel view image.

 

FAQ ID -1835
When should the N2 bottle used with the QIAxcel Advanced System be changed?

N2 cylinders on the QIAxcel Advanced should be changed when one or both low pressure warnings are shown in the "Status Information" panel.

The left panel of the screen in the "Process" environment is called the "Status Information" panel.  

"Pressure 1" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is sufficient for the current sample run; however, the N2 cylinder should be replaced once the run has finished.

"Pressure 2" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is insufficient for the current sample run and the analysis will not be performed. The N2 cylinder should be replaced.

FAQ ID -3001
How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?

The solutions used with the QIAxcel System or QIAxcel Advanced should not have to be changed for the life of the Gel Cartridge (based on the number of runs).  However, if any contamination is suspected, the Buffer Tray should be cleaned and the buffers should be changed immediately.

FAQ ID -1840
How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?

If the gel cartridge tips for the QIAxcel System and QIAxcel Advanced are in contact with air, the tips will dry out. There are 3 ways of preventing the tips from drying.

  1. Store the cartridge in the instrument with the instrument in the “Park” position. The washing solution (8 ml) covered with mineral oil must be in the “Park” position of the Solution Tray. 
  2. Store the cartridge in the Cartridge Stand with enough mineral oil in the well of the Cartridge Stand to cover the capillary tips. The Cartridge Stand should only be used for short term storage, i.e. when changing cartridges during one working day. 
  3. Place the cartridge back into its packaging with the capillary tips gently inserted into the soft gel in the box.
FAQ ID -1823
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?

The minimum sample volume required for the QIAxcel System and QIAxcel Advanced is 10 ul to guarantee sample injection in each channel.

 

 

FAQ ID -1852
How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?

If one of the capillaries of the gel cartridge for the QIAxcel System or QIAxcel Advanced is clogged, the corresponding lane in the gel-view image is typically black.

 

FAQ ID -1836
Can the QIAxcel System or QIAxcel Advanced be connected to a network?

Yes, the QIAxcel System or QIAxcel Advanced can be connected to a local network.

FAQ ID -1862
Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?

High or very low salt concentration could lead to the described appearance when running samples on the QIAxcel System and QIAxcel Advanced. Make sure QX DNA Dilution buffer has been used to dilute your samples. Dilute your samples 1:2 in QX DNA Dilution buffer and re-analyze.

FAQ ID -1837