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RNAprotect Tissue Reagent

For immediate stabilization of RNA in tissues
  • Immediate RNA stabilization and protection, due to RNase inactivation
  • Reliable gene expression and gene-profiling data
  • Convenient and safe handling at room temperature instead of freezing a sample
  • No need for liquid nitrogen or dry ice, ideal for sample collection in the field
  • Tissue archiving without risk of RNA degradation
RNAprotect Tissue Reagent immediately stabilizes RNA in tissue samples to preserve the gene expression profile. It is a storage solution that permeates harvested tissues rapidly to avoid freezing samples in liquid nitrogen. RNAprotect Tissue Reagent is supplied in 50 ml or 250 ml bottles. For stabilization of DNA, RNA, and protein in tissue samples, Allprotect Tissue Reagent can be used.

The following kits are compatible with the RNAprotect Tissue Reagent: RNeasy Plus Kits, RNeasy Kits, miRNeasy Kits, and AllPrep Kits.
Cat No./ID: 76104
RNAprotect Tissue Reagent (50 ml)
$86.10
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50 ml RNAprotect Tissue Reagent for stabilization of RNA in 25 x 200 mg tissue samples
Cat No./ID: 76106
RNAprotect Tissue Reagent (250 ml)
$359.00
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250 ml RNAprotect Tissue Reagent for stabilization of RNA in 125 x 200 mg tissue samples
The RNAprotect Tissue Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAGEN’s former RNA Stabilization Reagent, was manufactured and offered under license from Ambion, Inc., the owner of the RNAlater® trade mark. Following the expiration of that license and corresponding patents, QIAGEN’s new product name for the identical formulation is RNAprotect Tissue Reagent.
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RNAprotect Reagent prevents degradation of mRNA in tissues.
RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (Unstabilized) or RNeasy Protect Kits (Stabilized). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.
Performance
RNAprotect Tissue Reagent stabilizes and protects the RNA expression pattern of samples during harvest and storage (see figure "RNAprotect Reagent prevents degradation of mRNA in tissues"). Immediate protection with RNAprotect technology ensures that downstream analyses truly reflect the expression profile of the intact tissues. Samples can be archived without risk of RNA degradation, even after multiple freeze–thaw cycles. RNeasy purified RNA from RNAprotect stabilized samples stored under a wide variety of conditions is ideal for downstream processes such as northern blotting and RT-PCR.
Principle

Once a biological sample is harvested, its RNA becomes extremely unstable. The innovative, aqueous RNAprotect Tissue Reagent quickly permeates tissues resulting in immediate stabilization of the RNA and preservation of the RNA expression pattern. Gene induction or down-regulation triggered by sample manipulation is also prevented, allowing accurate analysis of gene expression.

Procedure

For RNA protection and sample storage, tissue samples are submerged in an appropriate volume of RNAprotect Tissue Reagent immediately after harvesting. RNAprotect Tissue Tubes provide premeasured volumes of RNAprotect Tissue Reagent in reclosable tubes for convenient handling and sample storage. The reagent preserves RNA for up to 1 day at 37°C, 7 days at 18–25°C, or 4 weeks at 2–8°C, allowing processing, transportation, storage, and shipping of samples without liquid nitrogen or dry ice. Alternatively, the samples can also be placed at –20°C or –80°C for archival storage.

Applications

Animal and human tissues stabilized in RNAprotect Tissue Reagent can be efficiently disrupted using QIAGEN instruments for sample disruption, and used with QIAGEN kits for:

  • Total RNA purification 
  • miRNA purification 
  • mRNA purification

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Kit Handbooks (1)
RNAprotect Tissue Tubes - For collection of harvested animal tissues with immediate stabilization of the gene expression profile, and subsequent transport and storage; RNAprotect Tissue Reagent - For immediate stabilization of the gene expression profile in harvested animal tissues
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