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NoviPure Soil Protein Kit

For the extraction of protein from all soil types
  • Efficient extraction of microbial protein from up to 5 grams of soil
  • Cleaner proteins with less humic substance interference
  • Improved downstream results with high-quality proteins
Get more proteins from your soil samples with less interference using the NoviPure Soil Protein Kit.

This novel kit extracts extracellular and intracellular microbial protein without co-extracting humic substances or other interfering compounds. The bead beating protocol uses a mixture of glass and ceramic beads along with two buffers to efficiently break down intracellular and extracellular protein. All materials are certified protein- and protease-free.

The resulting protein pellet has fewer impurities compared to other extraction methods and can be easily resuspended for further analysis or storage. Proteins are ready for 1D and 2D polyacrylamide gel electrophoresis and mass spectrometry (2D LC-MS/MS).

The NoviPure Soil Protein Kit was previously sold by MO BIO under the same name.

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Cat No./ID: 30000-20
NoviPure Soil Protein Kit (20)
$609.00
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For the extraction of protein from all soil types
The NoviPure Soil Protein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

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Figure 1. Pure protein extraction from soil with the NoviPure Soil Protein Kit.
C. albicans was added to 5 g of sterile compost samples. Protein was then extracted from those samples. Samples processed using the NoviPure Soil Protein Kit show greater protein extraction compared to the sucrose and phenol methods.
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Figure 2. High yields of protein from all soil types with the NoviPure Soil Protein Kit.
Challenging soil types still yield high amounts of protein using the NoviPure Soil Protein Kit. The kit was used to extract total protein from 5 g samples of sterile soil and sediments spiked with E. coli. To visualize protein results, 20 µl of each sample was processed using a stain-free 1D SDS-PAGE gel. In all cases, protein bands were clear and matched the E. coli culture control.
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Figure 3. More protein with the NoviPure Soil Protein Kit.
The NoviPure Soil Protein Kit is successful in extracting high-quantity and quality protein, as shown in 2D gel electrophoresis. E. coli-laced 5 g sterile compost samples were processed with the NoviPure Soil Protein Kit. Results were visualized using silver staining following 2D SDS-PAGE, with a pH range of 3–10, using 20 µl per protein sample. Humic substances did not interfere with visualization of proteins.
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Figure 4. Improved protein results with the NoviPure Protein Kit.
Protein was extracted using the NoviPure Soil Protein Kit and analyzed by 2D LC-MS/MS, where total ion chromatogram data reveals base peaks of protein. Visualized here is fraction 4 of the SCX cation exchange. The NoviPure Soil Protein Kit extracted protein from 5 g of sterile compost samples spiked with E. coli. Digestion and peptide enrichment followed using reverse phase ion exchange. Comparing raw spectra results with the SwissProt protein database (March 2013 release), 1076 unique proteins were identified.
Performance


Specifications

Features
Specifications
Bead size 0.1 mm glass, 0.1 mm ceramic mix
Format Patent-pending two buffer extraction method
Sample size 2-5 grams
Sample types Processed all types of soils, sand, and sediment
Storage temperature Store at room temperature (15-30°C) Pre-chill Solution SP1 and Solution SP2 before use
Throughput 1-2 samples

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