GeneRead qPCR SYBR® Green Mastermix

For use with the GeneRead Library Quant System

Features

  • Specific products with minimal primer–dimers
  • Instrument-specific all-in-one mixes
  • Available with ROX or fluorescein

Useful Resources

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GeneRead qPCR SYBR Green/Fluor Mastermix (2)

Cat. No. / ID: 180830

Two (2) tubes each containing 1.35 ml of 2x solution, sufficient for 100 standard 25 µl reactions
$245.00
Reference dye
Fluorescein
No dye
ROX
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GeneRead qPCR SYBR® Green Mastermix are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

GeneRead qPCR SYBR Green Mastermixes contain all of the optimized reagents and buffers needed for SYBR Green real-time polymerase chain reactions in all real-time PCR instrumentation. Each master mix includes real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and a reference dye (ROX or fluorescein) if and when needed to normalize specific instruments' optics. Simply add the master mix to PCR tubes along with template and primers. The chemically-modified and tightly controlled HotStart enzyme uniquely provides more accurate SYBR Green results by preventing the amplification of primer dimers and other nonspecific products.

Principle

GeneRead qPCR SYBR Green Mastermixes contain real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and SYBR Green dye. Mastermixes contain either fluorescein or ROX dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate SYBR Green results by preventing the amplification of primer–dimers and other nonspecific products.

Mastermixes with fluorescein 
GeneRead qPCR SYBR Green Fluor Mastermix is highly suited for real-time PCR applications using SYBR Green detection on instrumentation that uses fluorescein as a reference dye, including the Bio-Rad models iCycler, iQ5, MyiQ, and MyiQ2.

Mastermixes with ROX 
GeneRead qPCR SYBR Green ROX Mastermix is highly suited for real-time PCR applications using SYBR Green detection on instrumentation that uses ROX as a reference dye, including the Applied Biosystems models 5700, 7000, 7300, 7500 (Standard and Fast), 7700, 7900HT (Standard and Fast 96-well block, 384-well block), StepOnePlus, ViiA 7 (Standard and Fast 96-well block, 384-well block); Eppendorf Mastercycler ep realplex with or without ROX filter set; Stratagene models Mx3000P, Mx3005P, Mx4000; and Takara TP-800. 

GeneRead qPCR SYBR Green ROX FAST Mastermix 
GeneRead qPCR SYBR Green ROX FAST Mastermix is optimized for use with a fast real-time PCR protocol without compromising specificity or sensitivity, using SYBR Green detection. qBiomarker SYBR Green ROX FAST Mastermix is highly suited for use with QIAGEN Rotor-Gene Q, QIAGEN Rotor-Gene 6000 (Corbett), ABI, Eppendorf, Agilent/Stratagene, and TaKaRa instrumentation.

Applications

GeneRead qPCR SYBR Green Mastermixes are highly suited for real-time applications with SYBR Green, especially the GeneRead DNAseq Library Quant Array and the GeneRead Library Quant System.

Resources

Brochures & Guides (2)
Accelerate your NGS performance through Sample to Insight solutions
Introducing QIAseq
PDF (450KB)
Accelerate your NGS performance through Sample to Insight solutions