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RT2 qPCR Primer gDNA Controls

For detection of genomic DNA contamination in RNA and cDNA

Features

  • Sensitive detection of non-transcribed genomic DNA
  • For multiple species
  • Compatible with RT² qPCR Primer Assays

Useful Resources

Products

RT2 qPCR Primer gDNA Controls are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
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RT2 qPCR Primer gDNA Control (200)

Cat. No. / ID: 330011

RT2 qPCR Primer gDNA Contam. Control

Product Details

RT² qPCR Primer gDNA Controls specifically detect non-transcribed genomic DNA contamination with high sensitivity. RT² qPCR Primer gDNA Controls are available for human, mouse, rat, dog, and Rhesus macaque model systems. Each control vial contains sufficient primer for 200 PCR reactions.

Principle

RT² qPCR Primer gDNA Controls specifically detect a sequence of non-transcribed human, mouse, rat, dog, or Rhesus macaque genomic DNA with high sensitivity. This control helps to detect genomic DNA contamination in cDNA samples before characterization by SYBR® Green-based real-time PCR in order to prevent false-positive signals. RNA samples found to contain genomic DNA contamination may be treated with an appropriate source of RNAse-free DNAse and column purified before retesting with the RT² qPCR Primer gDNA Controls. Samples lacking genomic DNA contamination will provide accurate results in gene expression analysis.

Resources

Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Instrument Technical Documents (1)
For pathway-focused gene expression analysis

FAQ

How important is the RNA purification process, for obtaining reliable qRT-PCR results?

The most important prerequisite for any gene expression analysis experiment is the preparation of consistently high-quality RNA from every experimental sample. Contamination by DNA, protein, polysaccharide, or organic solvents can jeopardize the success of an experiment.

Genomic DNA contamination in an RNA sample compromises the quality of gene expression analysis results. The contaminating DNA inflates the OD reading of the RNA concentration. It is also a source of false positive signals in RT-PCR experiments.

RNase contamination degrades RNA samples whichcauses low signal and false-negative results in PCR.

Residual polysaccharides, collagen, other macromolecules, and organic solvents in an RNA sample can inhibit the activity of DNase, which may interfere with DNase treatment for genomic DNA removal. These contaminants may also inhibit reverse transcriptase and DNA polymerase, leading to lower reverse transcription efficiency and reduced PCR sensitivity.

For fast purification of high-quality RNA we recommend QIAGEN’s RNeasy Kits like the RNeasy Mini Kit, the RNeasy Plus Universal Kit, or the RNeasy FFPE Kit.

FAQ ID -2655