Nuclease-Free Water

For use in any molecular biology application


    Nuclease-Free Water (5 liters)

    Cat. No. / ID: 129117

    5 liters nuclease-free water prepared without the use of diethylpyrocarbonate (DEPC); Provided in five 1 liter bottles, delivered in a cardboard box.
    5 liters
    10 x 50 ml
    1000 ml
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    The Nuclease-Free Water is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

    Product Details

    Nuclease-Free Water is pure, quality-tested water suitable for use in all experiments that require nuclease-free water, including molecular biology applications. Nuclease-Free Water is prepared in a proprietary process, which yields DNase, RNase, and nuclease-free, deionized water without the use of chemical additives, such as diethylpyrocarbonate (DEPC). Nuclease-Free Water is provided in nuclease-free containers.

    Nuclease-Free Water is no longer available in a 5 liter bag with luer cap (cat. no. 129117). The required amount is supplied in five 1 liter bottles.


    Kit Handbooks (2)
    Highly pure, nuclease-free water for use in all molecular biology applications


    What quality level does your Nuclease-Free Water have?

    Nuclease-Free Water has been prepared without the use of chemicals such as DEPC (diethylpyrocarbonate) using an in-house method. The high quality of the water is assured by testing for DNase, RNase, and microbial contamination during the production process.

    FAQ ID -1292
    Is your Nuclease-Free Water fluorescence-free?

    Yes, QIAGEN's Nuclease-Free Water is distilled water that is completely free of substances that may fluoresce.


    FAQ ID -1291
    What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?

    cDNA generated with the miScript Reverse Transcription Kit can be diluted either with Nuclease-Free Water or TE buffer.



    FAQ ID -1601
    What is the pH value of QIAGEN's Nuclease-Free Water?

    At 22°C, Nuclease-Free Water has a pH value of between 5.0 and 6.5. It is not possible to determine the pH of highly pure water exactly. Therefore, many publications/industry standards do not provide a pH specification for highly pure water. Highly pure water does not contain enough ions or impurities for an exact pH determination. In general, values between pH 5 and 8 are obtained.

    FAQ ID -1290