ipsogen RUNX1-RUNX1T1 Kit

For quantitative detection of RUNX1-RUNX1T1 (or AML1-ETO) fusion gene transcripts using real-time PCR

Features

  • Sensitive quantitative assay
  • Accurate quantification relative to ABL control gene expression
  • EAC standardized assay for reproducible results
  • Optimal quality control and ISO 13485 certification
  • Easy workflow with ready-to-use solutions

Useful Resources

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ipsogen RUNX1-RUNX1T1 Kit (24)

Cat. No. / ID: 675013

For 24 reactions: ABL Control Gene Standards, RUNX1-RUNX1T1 Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix RUNX1-RUNX1T1 Fusion Gene
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The ipsogen RUNX1-RUNX1T1 Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The ipsogen RUNX1-RUNX1T1 Kit provides reagents optimized for reliable and sensitive detection and quantification of RUNX1-RUNX1T1 (or AML1-ETO) fusion gene transcripts in bone marrow or peripheral blood samples, relative to ABL control gene expression.

Performance

To ensure highest sensitivity, the ipsogen RUNX1-RUNX1T1 Kit has been optimized to detect ABL and RUNX1-RUNX1T1 fusion gene transcripts over a wide dynamic range (see figure " Sensitive detection of RUNX1-RUNX1T1 and ABL standards").

See figures

Principle

The ipsogen RUNX1-RUNX1T1 Kit is a ready-to-use kit for detection and quantification of RUNX1-RUNX1T1 fusion gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific RUNX1-RUNX1T1 fusion gene transcripts, relative to ABL control gene expression in total RNA (see figures " RUNX1-RUNX1T1 fusion gene transcript" and " ABL control gene transcript", and provides high levels of specificity, sensitivity, and reproducibility.
Each ipsogen RUNX1-RUNX1T1 Kit provides 3 standard dilutions for the ABL gene and 5 standard dilutions for the RUNX1-RUNX1T1 fusion gene. Primers and probe sets are designed according to Europe Against Cancer (EAC) recommendations.

See figures

Procedure

The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen RUNX1-RUNX1T1 Kit allows detection and quantification of RUNX1-RUNX1T1 fusion gene and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

The ipsogen RUNX1-RUNX1T1 Kit enables sensitive and reliable detection and quantification of RUNX1-RUNX1T1 fusion gene transcripts relative to ABL control gene expression for research use only.

Supporting data and figures

Resources

Kit Handbooks (1)
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.