DNA was purified from various tissues using the EZ1 DNA Tissue procedure. [A] PCR of the bovine obesity single-copy gene (850 bp fragment) using EZ1 purified DNA from bovine tissues. [B] PCR of GAPDH (266 bp fragment) using EZ1 purified DNA from 6 mouse tails. A 2.5 µl aliquot (5%) of each PCR was visualized by agarose gel electrophoresis. M: 100 bp marker; -C: negative control.