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Read more about GeneReader NGS System
The first complete Sample to Insight NGS solution.

QIAsymphony RGQ

Integrated automated sample preparation and assay setup, with manual transfer to the detection unit, for all molecular testing needs

Features

  • Unprecedented flexibility
  • Workflow optimization and utility
  • Maximized ease of use and convenience
  • Regulated assays and laboratory-developed tests
  • Designed for largest range of applications

Products

The QIAsymphony SP is intended to be used only in combination with QIAsymphony Kits indicated for use with the QIAsymphony SP for the applications described in the kit handbooks. If used in combination with QIAGEN kits indicated for use with the QIAsymphony AS instrument, the QIAsymphony AS is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAsymphony AS instrument is used with kits other than QIAGEN kits, it is the user's responsibility to validate the performance of such product combination for any particular application. The Rotor-Gene Q, if used in combination with QIAGEN kits indicated for use with the Rotor-Gene Q instrument, is intended for the applications described in the respective QIAGEN kits handbooks. If the Rotor-Gene Q instrument is used with kits other than QIAGEN kits, it is the user's responsibility to validate the performance of such product combination for any particular application.
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QIAsymphony Cabinet SP/AS

Cat. No. / ID: 9020246

Accessory for correct positioning of the QIAsymphony SP/AS instruments
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QIAsymphony RGQ, System

Cat. No. / ID: 9001850

QIAsymphony SP, QIAsymphony AS, Rotor-Gene Q 5plex HRM; includes required accessories and consumables, installation, and training; includes 1-year warranty on parts and labor

Product Details

QIAGEN's premier sample-to-result workflow solution comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and a broad range of ready-to-use assays on the Rotor-Gene Q real-time PCR cycler. QIAGEN continues to expand the QIAsymphony series with new features, assay menus, and workflows.

Explore the virtual demo to learn more about QIAsymphony RGQ.

Performance

The QIAsymphony RGQ, in combination with artus QS-RGQ Kits, enables highly sensitive and specific detection of:
See figures

Principle

QIAsymphony RGQ can save you time by processing up to 96 samples, from sample to result, in one integrated run. A dedicated range of kits enable open or closed systems for molecular testing.

QIAsymphony RGQ is the integrated system to maximize your efficiency and optimize your workflows, from sample to result. QIAsymphony RGQ synergizes the automated sample preparation and assay setup on the QIAsymphony SP/AS with manual transfer to the trusted Rotor-Gene Q for detection. The QIAsymphony SP automates sample preparation from a wide range of sample types. The QIAsymphony AS delivers highly reliable and reproducible PCR assay setup and is highly suited for laboratories that perform complex PCR-based molecular testing on a day-to-day basis. The QIAsymphony AS enables setup of multiple assays per run or sample and supports artus QS-RGQ products, for a complete CE-IVD compliant workflow, as well as in-house assays. The Rotor-Gene Q enables sensitive, precise real-time PCR analysis with CE-IVD-marked artus QS-RGQ Kits or in-house assays.

Kits for complete CE-IVD compliant workflow on the QIAsymphony RGQ
Kits Instrument Method
QIAsymphony DSP Virus/Pathogen Kits QIAsymphony SP Purification of viral nucleic acids or bacterial DNA from a broad range of sample types
artus QS-RGQ Kits QIAsymphony AS Real-time PCR (multiplex; sample and internal control)
Kits for in-house tests on the QIAsymphony RGQ
Application Kits Instrument Method
Purification QIAsymphony DSP AXpH DNA Kit QIAsymphony SP Purification of DNA from liquid-based cytology media
QIAsymphony DSP DNA Kits QIAsymphony SP Purification of DNA from human whole blood, buffy coat, tissues, FFPE tissues, cultured cells and bacterial cultures, as well as viral DNA from human whole blood
QIAsymphony DSP Virus/Pathogen Kits QIAsymphony SP Purification of viral nucleic acids or bacterial DNA from a broad range of sample types
QIAsymphony Certal Kits QIAsymphony SP Purification of residual host cell DNA and viral nucleic acids from bioprocess purification buffer, cell culture supernatant samples, and vaccine preparations
Assay setup — pathogen detection QuantiFast Pathogen +IC Kits QIAsymphony AS Multiplex real-time PCR and RT-PCR using sequence-specific probes
QuantiTect Virus Kits QIAsymphony AS Highly sensitive detection of multiple viral DNA and RNA targets in the same tube
QIAGEN OneStep RT-PCR Kit QIAsymphony AS Sensitive RT-PCR, with simple setup, using any RNA template
Assay setup — gene expression Rotor-Gene SYBR® Green Kits QIAsymphony AS Ultrafast, precise real-time PCR and RT-PCR using SYBR® Green I
Rotor-Gene Probe Kits QIAsymphony AS Ultrafast, precise real-time PCR and RT-PCR using sequence-specific probes
Rotor-Gene Multiplex Kits QIAsymphony AS Ultrafast, precise multiplex real-time PCR and RT-PCR using sequence-specific probes
Assay setup — residual DNA detection Certal Detection Kits QIAsymphony AS Duplex real-time PCR detection of resDNA target and a generic internal control

Procedure

The QIAsymphony RGQ workflow starts with purification of nucleic acids on the QIAsymphony SP instrument. The eluates, containing purified nucleic acids from the sample preparation procedure, are transferred to the QIAsymphony AS module, which performs assay setup. The assays are then manually transferred to the Rotor-Gene Q for PCR and data analysis.

To suit individual workflow requirements, there are 2 modes of system operation: integrated or independent. In the integrated mode, samples processed on the QIAsymphony SP are transferred automatically to the QIAsymphony AS, reducing manual handling steps and documentation. For added flexibility, the QIAsymphony SP and AS can also be operated independently of each other in independent mode. See " QIAsymphony workflows".

See figures

Applications

The QIAsymphony RGQ is the most versatile system for all molecular testing needs — today and tomorrow. The QIAsymphony RGQ allows consolidation of commerical assays, as well as in-house–developed tests, on a single platform.

The broad range of applications for open and closed systems includes:

  • artus transplantation panel (CMV, EBV, HSV, BK virus, and VZV)
  • artus blood-borne virus panel (HIV, HCV, and HBV)
  • In-house tests

Supporting data and figures

Specifications

FeaturesSpecifications
Elution volumeQIAsymphony SP — 30–500 µl (application dependent)
ProcessingQIAsymphony SP/AS — automated
Storage temperatureQIAsymphony SP/AS — 5–40°C (41ºF to 104ºF) in manufacturer's package Rotor-Gene Q — 15 to 30ºC (59ºF to 86ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 1K2 (IEC 60721-3-1)
FeaturesRotor-Gene Q — dynamic range, 10 orders of magnitude
Operating temperatureQIAsymphony SP/AS — 15–32°C (59–89.6ºF) Rotor-Gene Q — 18–30ºC (64–86ºF)
HumidityQIAsymphony SP/AS — relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C Rotor-Gene Q — relative humidity of 10–75% (noncondensing)
Pollution levelQIAsymphony SP/AS — 2 Rotor-Gene Q — 2; Environmental class 3K2 (IEC 60721-3-3)
Overvoltage categoryII
Samples per run (throughput)QIAsymphony SP — 1–96 samples in batches of 24 Rotor-Gene Q — tubes 0.2 ml; strip tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; up to 100 samples per run using a Rotor-Disc 100
Heat dissipation/thermal loadRotor-Gene Q — average, 0.183 kW (632 BTU/hour); peak, 0.458 kW (1578 BTU/hour)
Protocols/main application on this instrumentQIAsymphony SP — purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials QIAsymphony AS — PCR setup Rotor-Gene Q — gene expression analysis, microRNA detection, virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM)
TechnologyQIAsymphony SP — QIAGEN magnetic-particles chemistry Rotor-Gene Q — real-time PCR cycler
Input volumeQIAsymphony SP — minimum input volume: 200 µl (application dependent)
Optical SystemRotor-Gene Q — up to 6 channels spanning UV to infra-red wavelengths; excitation sources: high energy light-emitting diodes; detector: photomultiplier; acquisition time: 4 s; the software allows to create new excitation/detection wavelength combinations
Kits or Application Packs designed for this instrumentQIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits Rotor-Gene Q — artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit
Place of operationFor indoor use only
PowerQIAsymphony SP/AS — 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages Rotor-Gene Q — 100–240 V AC, 50–60 Hz, 520 VA (peak); Power consumption 8 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5A 250 V fuse
SoftwareRotor-Gene Q — Rotor-Gene Q software, supplied on the provided installation CD
Thermal performanceRotor-Gene Q — temperature range, 35 to 99ºC; temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); temperature resolution, ±0.02ºC (smallest programmable increment); temperature uniformity, ±0.02ºC; ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling
Typical run timeRotor-Gene Q — 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent)
WeightQIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg; Rotor-Gene Q, 12.5 kg (27.6 lb.) (standard configuration)
Transportation conditionsQIAsymphony SP/AS — –25 to 70°C (–13ºF to 158ºF) in manufacturer's package Rotor-Gene Q — –25 to 60ºC (–13ºF to 140ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 2K2 (IEC 60721-3-2)
WarrantyRotor-Gene Q — 1 year on instrument; lifetime guarantee on excitation LEDs
Instrument dimensionsQIAsymphony SP/AS — QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm Rotor-Gene Q — width, 37 cm (14.6 in.); height, 28.6 cm (11.3 in.); depth (without cables), 42 cm (16.5 in.); depth (door open), 53.8 cm (21.2 in.)
AltitudeUp to 2000 m (6500 ft)

Service Plans

QS RGQ, Premium Agreement

Cat. No. / ID: 9242764

On-site QIAsymphony RGQ instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one Preventive Maintenance or Inspection Service during the Premium Agreement period.

Resources

Application/Protocol Documents (29)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
Supporting Software and Tools (2)
For QIAsymphony software version 5.0.4
Russian language package for QIAsymphony software version 5.0.4
Protocol Files (7)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
Instrument User Manuals (3)
For Rotor-Gene Q instruments using Rotor-Gene Q Software version 2.3.4
Guideline for ISO 20836:2020
For use with Rotor-Gene Q Software version 2.3.4
Instrument Technical Documents (9)
QIAsymphony Cabinet SP/AS documents
Differences between QIAsymphony software versions
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony Cabinet SP/AS document
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
Software document for QIAsymphony software version 4.0.1
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
Labware Files and Documents (2)
Labware package SOW-516-0-08 for use with the QIAsymphony SP/AS instruments (software version 5.0)
Brochures & Guides (1)

FAQ

The QIAsymphony RNA kit uses RLT plus as lysis buffer. Can samples lysed in RLT and then stored in the freezer be used on the QIAsymphony?

The RLT buffer is more viscous than the RLT plus buffer. Extraction will work fine, but there may be some magnetic bead carryover to the eluate.

FAQ ID - 3533
There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do?

The barcode on the adapter at the cooling elution position is read by default. This can be changed in the configuration settings which are available from the tools menu if you have logged in as Supervisor. The option is on the tab Process SP1 and is called ”On which elution slots should the bar codes be scanned”. To disable the barcode reading, put the setting to ”none”.

FAQ ID - 3532
What is the elution volume for all CE-marked artus QS-Rotor-Gene Q assays?
The elution volume for all artus QS-RGQ assays is 60µl.
FAQ ID -2432
What is the batch size of the QIASymphony Rotor-Gene Q and how many PCR´s can be processed per assay module run?

With the QS-RGQ the user can process 1 to 4 batches with 1 to 24 reactions per specific batch. RNA assays are limited to 72 reactions in total.

For PCR:  216 reactions for DNA and 72 reactions for RNA viruses can be performed.

FAQ ID -2433
Is barcode reading supported by QS-RGQ?
Yes. The procedure in more detail is described in the instrument handbook.
FAQ ID -2437
Is it possible to process samples on the AS-module while SP is running?
Yes. A connected as well as an independent operation between QS-SP and QS-AS is possible.
FAQ ID -2435