High-performance SYBR® Green-based detection
QuantiNova LNA PCR Flexible Panels use SYBR® Green-based detection, which, when used with a highly specific PCR system, well-designed primers and optimized reaction conditions, offers several advantages over hydrolysis probe detection. First, it is possible to perform melting curve analysis immediately following the PCR, so you can confirm high specificity of the reaction and rule out formation of primer–dimers or non-specific amplicons. It also provides higher flexibility in the assay design, because you only need two primers, instead of two primers and a probe. Furthermore, while the primers ensure the specificity of the detection, the dye’s binding properties enable analysis of many different targets without having to synthesize target-specific, labeled probes, reducing your assay setup and running costs.
Choose from assays on our cataloged Focus Panels
QuantiNova LNA PCR Focus Panels include the assays needed for a thoroughly researched panel of relevant pathway- or disease-focused genes. The predesigned assays have been designed using our proprietary algorithm to provide the most sensitive, accurate and effective analysis. Most of the assays are intron-spanning when possible and detect only RNA. Assays that do not span an intron are designated as such, and if there is one exon in the target, unwanted signals can be easily eliminated using the QuantiNova Reverse Transcription Kit with the integrated gDNA removal step.
Flexible Panel vs. Custom Panel
QuantiNova LNA PCR Flexible Panels are a semi-customizable product and are available in 96-well plate, 384-well plate and 100-well disc formats, with up to 96 different assays on each plate. Flexible Panels can only contain assays from our available Focus Panels. Our convenient custom builder allows you to simply load the plate content of an existing Focus Panel and swap out the assays to meet your requirements. Reference gene assays and controls from our Focus Panels can also be added.
QuantiNova LNA PCR Custom Panels are a fully customizable product and are also available in 96-well plate, 384-well plate and 100-well disc formats, with all of our different plate layouts available. The custom builder allows you to upload your own list of targets and add any predesigned or custom assays of your choice. Any reference genes and control assays and controls can also be added.
Available plate layouts, minimum order quantity and multiple plate discount
|Plate format ||Number of different assays per plate ||Configuration ||Minimum order |
|96-well or 100-well disc ||8 assays ||8 assays x 12 samples ||4 plates |
|12 assays ||12 assays x 8 samples ||4 plates |
|16 assays ||16 assays x 6 samples ||4 plates |
|24 assays ||24 assays x 4 samples ||4 plates |
|32 assays ||32 assays x 3 samples ||4 plates |
|48 assays ||48 assays x 2 samples ||4 plates |
|96 assays ||96 assays x 1 sample ||6 plates |
|384-well ||8 assays ||8 assays x 48 samples ||6 plates |
|12 assays ||12 assays x 32 samples ||6 plates |
|16 assays ||16 assays x 24 samples ||6 plates |
|24 assays ||24 assays x 16 samples ||6 plates |
|32 assays ||32 assays x 12 samples ||6 plates |
|48 assays (horizontal) ||48 assays x 8 samples ||6 plates |
|48 assays (normal vertical) ||48 assays x 8 samples ||6 plates |
|64 assays ||64 assays x 6 samples ||6 plates |
|96 assays ||96 assays x 4 samples ||6 plates |
|128 assays ||128 assays x 3 samples ||not applicable |
|192 assays ||192 assays x 2 samples ||not applicable |
|384 assays ||384 assays x 1 sample ||not applicable |
|Plate quantity ordered ||Discount (%) |
|1–5 ||0 |
|6–11 ||15 |
|12–23 ||30 |
|24+ ||50 |
Complimentary data analysis
The complimentary, web-based data analysis tool in the GeneGlobe Data Analysis Center includes a user-friendly wizard to guide you step-by-step through the normalization and analysis of your data and generates publication-ready results figures.