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QIAvac Holder

Cat. No. / ID: 19418

Holder for stabilizing QIAGEN Plasmid Plus Mega and Giga columns with Tube Extenders on the QIAvac 24 Plus
Accessories
QIAvac Holder
QIAvac Luer Adapter Set
VacConnectors
Vacuum Pump
VacValves
Vacuum Regulator

Product Details

The QIAvac Accessories are supplemental materials for processing 96-well plates or columns.
The QIAvac accessories have to be used only together with QIAvac 24 Plus.

Performance

The QIAvac Vacuum Manifolds facilitate sample handling by providing a convenient modular manifold system for processing QIAvac multiwell modules or columns.

Recommended use of QIAvac Accessories
FormatModule processedQIAvac model Accessories
96-well plates QIAGEN Plasmid Plus 96, QIAprep 96 Plus, QIAprep 96 Turbo, QIAquick 96, R.E.A.L. 96, RNeasy 96 QIAvac 96 Vacuum Regulator (optional)
DirectPrep 96 MiniPrep Kit, MinElute 96 UF PCR Purification Kit QIAvac Multiwell
Spin columns QIAprep, MinElute, QIAquick, RNeasy, QIAGEN Plasmid Plus Midi, QIAGEN Plasmid Plus Maxi, CompactPrep Midi, CompactPrep Maxi QIAvac 24 Plus
or
QIAvac 6S* QIAvac Luer Adapters
QIAamp Mini (where vacuum protocol is available)  QIAvac 24 Plus VacConnectors
VacValves (optional)
or
QIAvac 6S* QIAvac Luer Adapters
VacConnectors
QIAamp Midi, QIAamp Maxi, QIAamp DSP QIAvac 24 Plus VacConnectors
VacValves (optional)
QIAvac Connecting System
QIAGEN Plasmid Plus Giga, QIAGEN Plasmid Plus Mega QIAvac 24 Plus QIAvac Holder
* No longer available.
Not available in all countries; please inquire. 

Principle

The QIAGEN Vacuum Pump is suitable for all standard laboratory vacuum procedures (capacity 34 L/min, 8 mbar vacuum abs.). The Vacuum Pump, in combination with QIAvac vacuum manifolds, enables vacuum processing of many QIAGEN nucleic acid purification kits. Vacuum-driven liquid processing is an attractive alternative to centrifugation that minimizes the hands-on time needed for sample preparation. The QIAvac Connecting System together with the QIAvac 24 Plus vacuum manifold has been used successfully evaluating kit performance at QIAGEN.

The QIAvac Holder (see figure " QIAvac Holder") is designed to stabilize the QIAGEN Plasmid Plus Mega and Giga columns together with Tube Extenders on the QIAvac 24 Plus. It prevents movement of the assembled columns and extenders, particularly when containing large volumes of liquid.

VacValves are inserted directly into the luer slots of the QIAvac 24 Plus manifold and should be used if sample flow rates differ significantly to ensure consistent vacuum. VacValves ensure constant vacuum pressure when using viscous samples, such as blood.

VacConnectors (see figure " VacConnector") are disposable connectors that fit onto the outlet nozzles of QIAamp spin columns and QIAprecipitator modules, and into the luer extensions of QIAvac 24, QIAvac Luer Adapters on QIAvac 6S (no longer available), or VacValves on QIAvac 24. They enable efficient vacuum processing as an alternative to microcentrifugation for isolation of nucleic acids. VacConnectors are designed to prevent direct contact between spin columns or modules and luer connectors during purification, avoiding even trace amounts of cross-contamination. VacConnectors are discarded after single use.

QIAvac Luer Adapters allow convenient processing of up to 24 spin columns on QIAvac 6S (no longer available). Samples and wash solutions are drawn through the spin columns by vacuum rather than centrifugation, providing even greater speed in purification procedures. The outlet nozzles of QIAprep, QIAquick, and MinElute spin columns have been designed to fit directly into the luer connectors on QIAvac Luer Adapters. Each Luer Adapter contains 4 luer connectors and six Luer Adapters fit into the slots of the QIAvac 6S top plate, allowing 24 samples to be processed at once. Use of the VacConnectors in addition to QIAvac Luer Adapters enables QIAamp spin columns to be accommodated on QIAvac 6S (no longer available).

The Vacuum Regulator (see figure " Vacuum Regulator") supplied by QIAGEN allows easy monitoring and adjustment of the vacuum pressure for procedures using QIAvac manifolds. Optimal yield and purity of nucleic acid preparations are obtained when the pressure generated by the vacuum source is within a specific range for the QIAGEN purification chemistry being used (as indicated in each product handbook).

See figures

Procedure

For procedures, please refer to the handling guidelines in the corresponding handbook.

Applications

QIAvac Accessories facilitate vacuum processing of samples for various applications.

Supporting data and figures

Resources

User-Developed Protocols (3)
The procedure has been used successfully for isolation of a single-copy, 14.5 kb, binary plasmid, p35S GUS INT, from Agrobacterium tumefaciens strain GV2260 (1).
This procedure has been adapted by customers and is for purification of viral RNA and DNA from plasma, serum, and cell-free body fluids using the QIAamp MinElute Virus Vacuum Kit.

FAQ

Does the QIAamp DSP Virus Kit require the QIAvac 24 Plus, or can I use it with my own laboratory vacuum system?
It is possible to use a general laboratory vacuum system with the QIAamp DSP Virus Kit. However, this will require validation in-house. The CE-marked QIAamp Kits have been developed, field-tested and validated with the QIAvac 24 Plus vacuum system (QIAvac 24 Plus, QIAvac Connecting System and Vacuum Pump). Using this system with the QIAamp DSP Virus Kit will avoid lengthy validation procedures for the enduser.
FAQ ID -789