QIAamp DNA Blood Kits simplify DNA purification from blood and DNA purification from body fluids with fast spin-column, vacuum, centrifugation or automated procedures (see figure “ QIAamp Spin Column procedure”). Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA and heparin, may be processed. If processing QIAamp Midi or Maxi spin columns on vacuum manifolds additional Buffer AW1 [cat. no. 19081] and Buffer AW2 [cat. no. 19072] are required for use with the vacuum manifolds.
Vacuum processing with the QIAamp DNA Blood Mini Kit
With the QIAamp DNA Blood Mini Kit, blood can be processed by vacuum instead of centrifugation, for greater speed and convenience in DNA purification. QIAamp mini spin columns are accommodated on the QIAvac 24 manifold using VacValves and VacConnectors. VacValves should be used if sample flow rates differ significantly, to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination. Use of VacConnectors also allows these QIAamp spin procedures to be performed on QIAvac 6S with QIAvac Luer Adapters.
Automated processing on the QIAcube Connect
The award-winning QIAcube Connect uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into laboratory workflows. All steps in the purification procedure are fully automated – up to 12 samples can be processed per run. The QIAcube Connect used together with the dedicated QIAamp DNA Mini QIAcube Kit provides a winning combination for fast, easy and convenient DNA purification.
High-throughput processing in 96-well format
The QIAamp 96 spin procedure requires the QIAGEN 96-Well-Plate Centrifugation System’s deep rotor buckets to accommodate QIAamp 96 plates stacked on 96-well blocks. Fresh or frozen whole blood treated with common anticoagulants such as, EDTA, citrate and heparin, may be used. Dried whole blood may be processed with additional equipment and the use of a special protocol available from QIAGEN Technical Services or your local distributor.