The Microbial DNA-Free Water is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Microbial DNA-Free Water is molecular biology grade, nuclease-free water that has been filter-sterilized and UV-treated to eliminate genomic DNA contamination. Quality of the water is tested by PCR using a panel of Microbial DNA qPCR Assays that are sensitive to bacterial and fungal DNA. If none of the PCR assays give a positive signal using Microbial DNA-Free Water as the template in the PCR reaction, then the lot passes. This rigorous process ensures the cleanest possible results for Microbial DNA qPCR Assay or Array experiments.
Quality control is performed on each lot of Microbial DNA-Free Water to verify the absence of microbial DNA. The result of the quality control test is included on the product sheet that accompanies the product.
A stringent purification and testing process ensures that Microbial DNA-Free Water is clean and entirely free of genomic DNA contamination. The water is sterilized using a 0.22 μl filter, and UV treatment eliminates genomic DNA. Once the purification process is complete, each lot of water is quality control tested for fungal or bacterial DNA contamination using Microbial DNA qPCR Assays, and must give a negative signal for each assay in order to receive a passing score.
Microbial DNA-Free Water is highly suited for use with Microbial DNA qPCR Arrays and Assays as a diluent for concentrated Microbial DNA Positive Control and Microbial qPCR Mastermix, and as a no-template control.
For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes
What sequences are used to design the Microbial DNA qPCR Assays?
The Microbial DNA qPCR Assays for bacterial species detection use the 16S rRNA gene as the template. Microbial DNA qPCR Assays for detection of fungal species use ribosomal (small subunit or larger subunit) genes as the template. Microbial DNA qPCR Assays for detection of microbial genes use various antibiotic resistance genes and virulence factor genes for the template.
FAQ ID — 3403
What are the storage conditions for the Microbial DNA qPCR products?
Store Microbial DNA qPCR Array plates at room temperature.
Store Microbial DNA qPCR Assays, Microbial DNA qPCR Assay Kits, and Microbial DNA qPCR Multi-Assay Kits at –20°C.
Store Microbial qPCR Mastermix at –20°C.
Store Microbial DNA Positive Control at –20°C.
Store Microbial DNA-Free Water at –20°C.
FAQ ID — 3394
What is the difference between LLOQ and LOD?
Limit of detection (LOD) is defined as the lowest concentration at which 95% of positive samples are detected. Since LOD is not necessarily within the linear range of an assay, LOD can be lower than LLOQ.
FAQ ID — 3402
Can I measure antibiotic resistance gene expression?
The Microbial DNA qPCR Arrays and Assay Kits do not allow measurement of antibiotic resistance gene expression because reverse transcriptase enzyme is not included. Microbial DNA qPCR Assays have not been tested on cDNA.
FAQ ID — 3404
What is LLOQ?
The lower limit of quantification (LLOQ) is the lower limit at which an assay can provide quantitative results. It is the lowest template concentration that is within the linear range of the assay.
FAQ ID — 3401
Are the assays species-specific?
Most of the Microbial DNA qPCR Assays that detect bacterial species are species-specific. Assays that do detect more than one species will list which species are detected in the product specification sheet. These were determined by both experimental data and in silico prediction.
FAQ ID — 3400
Which Microbial qPCR Mastermix should I use?
The Microbial qPCR Mastermix (ROX) is suitable for use with the following real-time cyclers: all Applied Biosystems and Agilent (formerly Stratagene); BioRad Opticon, Opticon 2, and Chromo 4; Roche LightCycler 480; and Eppendorf Mastercycler ep realplex 2, 2S, 4, and 4S.
The Microbial qPCR Mastermix (Fluorescein) is suitable for use with the following real-time cyclers: BioRad iCycler, MyiQ, and iQ5.