Aliquots of 200 mg stool sample from different animals were processed on the QIAcube HT instrument (blue bars) using the QIAamp 96 PowerFecal QIAcube HT Kit, or manually using the QIAamp Fast DNA Stool Mini Kit (pink bars). Real-time PCR of 16S rRNA gene of Corynebacterium glutamicum was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 1 µl eluate in a 20 µl reaction. DNA extracted either manually or on the QIAcube HT instrument showed similar performance for all the stool samples.
Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR was carried out on the Rotor-Gene Q using the QuantiTect Virus PCR Kit with 2 µl and 10 µl eluates respectively, from each sample in 20 µl reactions containing an internal control. CT values were compared to a standard containing no sample but only internal control (dark blue bar). All the three samples either extracted on different instruments or manually showed similar performance compared to the standard, regardless of the volume of eluate used, indicating no PCR inhibition.
Nucleic acids were purified from serial dilutions of plasma samples spiked with a typical RNA or DNA virus. Samples were processed using QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol and were analyzed using in-house PCR and RT-PCR assays. The percentage of positive samples at low virus titers is shown. The resulting 95% probit values were 316.84 IU/ml for the RNA virus and 18.54 IU/ml for the DNA virus.
Various sample types were spiked with 20,000 IU of a typical DNA virus. Viral DNA was purified from 200 µl of each sample lysate using the QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol. Purified viral DNA was detected using an in-house PCR assay with 20 µl each eluate per reaction.
Viral nucleic acids were purified from 200 µl plasma samples spiked with 10,000, 1000 and 100 IU/ml of a typical DNA or RNA virus. Sample processing was automated using either QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol, QIAxtractor with the VX Protocol, or QIAcube with the QIAamp MinElute Virus Spin Kit. Viral nucleic acids were detected using in-house PCR and RT-PCR assays, with 20 µl eluate per reaction on the Rotor-Gene Q. Results show that QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit performs as well as or better than the other methods.