Investigator Argus Y-28 QS Kit

For powerful discrimination in Y-chromosomal analyses with an innovative Quality Sensor

Features

  • 6-dye chemistry for analysis of 27 YChr STR markers, of which 6 are rapidly mutating (RM)
  • Integrated performance control using Quality Sensor technology optimized for ≥ 24 cycles
  • Fast Reaction Mix 3.0 for best-in-class sensitivity and robustness against inhibitors, even in high female backgrounds
  • Simplified workflow with a single kit for both purified DNA and reference samples
  • Reduced allelic overlap to minimize the risk of data misinterpretation
Investigator Argus Y-28 QS Kit (400)

Cat. No. / ID: 383627

For 400 reactions: Primer Mix, Fast Reaction Mix 3.0, Control DNA, Allelic Ladder, DNA Size Standard, Nuclease-Free Water
KitAccessories
Investigator Argus Y-28 QS Kit
Matrix Standard
Investigator STR GO! Lysis Buffer
Investigator STR GO! Punch Buffer
Samples
400
100
The Investigator Argus Y-28 QS Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.

Product Details

The Investigator Argus Y-28 QS Kit allows multiplex amplification of 27 Y-chromosomal STR markers from multiple male-specific sample types, such as male–male and male–female mixtures, as well as direct PCR amplification from single-source samples. Due to the choice of markers, of which six are rapidly mutating (RM), the kit offers best-in-class discrimination power. In addition, the kit includes the innovative Quality Sensor, which generates additional, valuable data for your quality control and performance checks. The kit uses QIAGEN's latest fast-cycling technology FRM 3.0, which not only enables PCR in around 90 minutes, but also provides enhanced stability and inhibitor tolerance. Highly sensitive, yielding rapid and reliable results from trace DNA, the Investigator Argus Y-28 QS Kit is suitable for Y-STR casework, database samples and paternity testing.

Performance

The Investigator Argus Y-28 QS Kit uses our latest FRM 3.0 technology and provides reliable results in 90 minutes from casework samples or, in a direct amplification approach, from reference samples. For amplification from purified single source samples, we also offer a fast cycling protocol that takes only ~70 minutes. This proven QIAGEN multiplex PCR technology has been optimized for ease of handling and increased sensitivity. The Investigator Argus Y-28 QS Kit is optimized to improve the resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a high background of female DNA, and offers laboratories both powerful discrimination and high performance to enable accurate results from challenging samples. Assay performance tests in the presence of increasing concentrations of inhibitors showed both high sensitivity and superior inhibitor resistance. This means reliable, high-quality results.
The Investigator Argus Y-28 QS Kit was validated and evaluated using the Veriti and GeneAmp 9700 thermal cyclers and the Applied Biosystems 3500 Genetic Analyzer. The recommended amount of DNA under standard conditions is 0.5 ng. Internal validations demonstrated robust and balanced results with 0.1 to 2 ng DNA, and reliable results with <0.1 ng DNA.

Principle

The Investigator Argus Y-28 QS Kit uses QIAGEN's fast-cycling PCR technology for the rapid, simultaneous amplification of 27 Y-chromosomal STR markers and the Quality Sensor. Carefully designed primers minimize the overlap of alleles of different makers, thereby minimizing the likelihood of data misinterpretation.
The innovative Quality Sensor provides additional information about the sample quality and PCR success beyond the profile of the sample. With two Quality Sensors amplified as a positive control, it is possible to determine:
• If the PCR amplification worked
• If there was any DNA in the sample
• If the sample was degraded
• If inhibitors in the sample prevented successful PCR
This additional information will greatly enhance how you analyze your data and aid you in making crucial decisions with respect to further sample processing. Ultimately, the Quality Sensor will help you reduce unnecessary re-runs, saving you time and money.

Procedure

The 27 Y-chromosmal STR markers, and the Quality Sensor, are amplified simultaneously in a single PCR and labeled with one of the dyes listed below in Table 1.

6-FAM™ DYS389-I DYS391 DYS389-II DYS533 DYS390 DYS643  
BTG DYS458 DYS393 DYS19 DYS437 DYS449    
BTY DYS460 DYS576 YGATAH4 DYS481 DYS448 DYS518  
BTR2 DYS439 DYS549 DYS438 DYS456      
BTP QS1 DYS570 DYS635 DYS385 DYS392 DYS627 QS2


The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X. See the kit technical specifications for more information.

Applications

The Investigator Argus Y-28 QS Kit allows rapid and reliable generation of DNA profiles from blood, buccal swabs and forensic stains from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.
It is highly suited for:
• Criminal database and forensic casework samples
• Paternity testing
• Population genetics studies
• Other human identification and forensics applications

Features
Investigator Argus Y-28 QS Kit
Investigator Argus Y-12 QS Kit
Investigator Argus X-12 QS Kit
Volume per PCR 25 µl 25 µl 25 µl
Matrix BT6 BT5 BT5
Fluorescence labels 6-FAM, BTG, BTY, BTR2, BTO, BTP 6-FAM, BTG, BTY, BTO 6-FAM, BTG, BTY, BTR, BTO
Genetic analyzers Applied Biosystems 3500/3500xL Genetic Analyzers ABI PRISM 310, ABI PRISM 3100/3100-Avant, and Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers ABI PRISM 310, ABI PRISM 3100/3100-Avant, and Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis  Applied Biosystems GeneMapper ID, GeneMapper ID-X or Genotyper Applied Biosystems GeneMapper ID, GeneMapper ID-X Applied Biosystems GeneMapper ID, GeneMapper ID-X

Supporting data and figures

Resources

Brochures & Guides (2)
Advanced sample collection, automation and STR solutions for kinship testing
Technical Information (4)
Best-in-class performance and powerful discrimination for Y-chromosomal analysis
Quality initiatives for human identity testing and forensics 
Quick-Start Protocols (1)
Template Files (3)
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
Additional Resources (1)