The influence of serum and DNA quantity on transfection using SuperFect Reagent was examined. COS-7 cells (2 x 104 per well) were seeded in 96-well plates one day prior to transfection. Cells were transfected using 0.1–2.0 µg beta-galactosidase-reporter plasmid and 3 µl SuperFect Reagent per well, in either the presence or absence of serum. Each bar represents the average efficiency from 4 replicates assayed 48 hours after transfection.
Expression of green fluorescent protein (GFP) in differentiated PC-12 cells was examined 5 days post-transfection. Cells (104–105) previously stimulated with 50 ng/ml NGF were plated per 60 mm dish one day prior to transfection. Transient transfections were performed in 2 ml low-serum growth medium (DMEM plus 0.05% FBS) using 3 µg of a GFP-reporter plasmid and 15 µl SuperFect Reagent. (Data kindly provided by K. Kelly-Spratt, University of Texas Southwestern Medical Center, Dallas, TX, USA.)