Note the highly branched structure of an activated dendrimer in this schematic representation.
Expression of green fluorescent protein (GFP) in differentiated PC-12 cells was examined 5 days post-transfection. Cells (104–105) previously stimulated with 50 ng/ml NGF were plated per 60 mm dish one day prior to transfection. Transient transfections were performed in 2 ml low-serum growth medium (DMEM plus 0.05% FBS) using 3 µg of a GFP-reporter plasmid and 15 µl SuperFect Reagent. (Data kindly provided by K. Kelly-Spratt, University of Texas Southwestern Medical Center, Dallas, TX, USA.)
A model of the PolyFect-DNA complex shows PolyFect Reagent (purple balls) interacting with DNA (black) to form a ring-like (toroid-like) structure. The upper right section of the illustration shows naked DNA, the lower section shows the interaction between dendrimers and DNA inside the complex, and the upper left section shows the final complete coverage of DNA within the complex.
The influence of serum and DNA quantity on transfection using SuperFect Reagent was examined. COS-7 cells (2 x 104 per well) were seeded in 96-well plates one day prior to transfection. Cells were transfected using 0.1–2.0 µg beta-galactosidase-reporter plasmid and 3 µl SuperFect Reagent per well, in either the presence or absence of serum. Each bar represents the average efficiency from 4 replicates assayed 48 hours after transfection.