Total RNA purified from Schizosaccharomyces pombe. Results presented as [A] a gel image and [B] a superimposed electropherogram view. Reliable quality control of unlabeled cRNA generated from yeast total RNA. Results presented as [C] a gel image and [D] a superimposed electropherogram view.
Total RNA was extracted from rat liver samples using the RNeasy Plus Mini Kit and subjected to decomposing conditions. Samples of increasing levels of degradation were analyzed on the QIAxcel Advanced and the Agilent 2100 Bioanalyzer platform. The results are depicted in the gel view pictures for the QIAxcel Advanced [A] and the Agilent Bioanalyzer [B]. The electropherogram of selected samples analyzed on the QIAxcel Advanced is shown in [C]. The RIS (QIAxcel Advanced) and RIN (Agilent Bioanalyzer) numbers are listed in the table.
Nucleic acid molecules are size separated by applying a current to a gel-filled capillary, and detected as they migrate toward the positively charged terminus. The signal data pass through a photomultiplier and are converted to an electropherogram and gel image by the QIAxcel ScreenGel Software.
QIAxcel ScreenGel software simplifies data analysis.
Electropherogram views of purified total RNA. Data kindly provided by D. van Leenen, Microarray Facility, University Medical Center Utrecht, The Netherlands.
QIAxcel ScreenGel software simplifies experiment setup and data analysis.
Samples were analyzed on either [A] the QIAxcel Advanced System at the original concentration or [B] by agarose gel electrophoresis with five-fold concentration. Samples 3 [C] and 5 [D] were analyzed by agarose gel electrophoresis at the original concentration (O), with five-fold concentration (C), and at the original concentration on the QIAxcel Advanced (Q). The arrows represent bands originally scored as negative. Data kindly provided by Mutschall and coworkers, Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta, Canada.
For automated analysis of DNA fragments using QIAxcel instruments
Nucleic acid separation on the QIAxcel Advanced System, using the QX DNA High Resolution Kit takes less than 10% of the time of conventional high-resolution agarose.
[A] Agarose gel photo showing gDNA in various states of degradation. Lane 1: Non-degraded gDNA samples; lanes 2 and 3: gDNA samples partially degraded by sonication with 7 and 14 pulses, respectively; lanes 4 and 5: gDNA samples fully degraded with 70 and 105 pulses, respectively. [B] QIAxcel gel photo showing gDNA samples in three states of degradation. 1, 3, and 5 correspond to lanes 1, 3, and 5 in [A]. [C] Electropherograms and a gel photo of gDNA samples from lanes 1, 3, and 5 in [A]. Lane 1: Electropherogram indicates very good quality gDNA (no degradation products before the major peak, long tailing off); lane 3: partially degraded gDNA (some degradation product signals, no tailing off); lane 5: highly degraded gDNA (many degradation product signals, no tailing off).