Investigator IDplex GO! Kit

For multiplex direct amplification of 13 CODIS core loci, plus D2S1338, D19S433, and Amelogenin from swabs and papers

Highly suitable for reference samples from buccal cells/blood on FTA paper or buccal swabs
Low price per result in a high throughput reference setup
Fastest direct amplification workflow in forensics and paternity analysis
Straightforward protocols with ready-to-use Fast Reaction Mix
Simultaneous amplification of 16 loci

The Investigator IDplex GO! Kit is a multiplex application for human identification covering the 13 CODIS (Combined DNA Index System) core loci, D2S1338, D19S433, and Amelogenin. The kit utilizes a modified version of QIAGEN’s fast-cycling plus technology, enabling amplification in as little as 45 minutes. With rapid and reliable results from reference samples such as buccal swabs, as well as buccal cells or blood on papers, the Investigator IDplex GO! Kit is suitable for reference testing in forensic applications and paternity testing.

Buy Products
Product Details
Product Resources

Buy Products

Cat No./ID: 381636

Investigator IDplex GO! Kit (200)

Inquire

Primer mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, allelic ladder IDplex GO!, DNA size standard 550 (BTO)

Cat No./ID: 381638

Investigator IDplex GO! Kit (1000)

Inquire

Primer mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, allelic ladder IDplex GO!, DNA size standard 550 (BTO)

Cat No./ID: 386516

Investigator STR GO! Lysis Buffer (200)

Inquire

Lysis buffer for 200 swab samples

Cat No./ID: 386526

Investigator STR GO! Punch Buffer (200)

Inquire

Lysis buffer for 200 samples of epithelial cells on paper

Cat No./ID: 386528

Investigator STR GO! Punch Buffer (1000)

Inquire

Lysis buffer for 1000 samples of epithelial cells on paper

The Investigator IDplex GO! Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease. This product is not available in all countries.

Product Details

Performance

The Investigator IDplex GO! Kit utilizes fast-cycling technology to provide reliable results in approximately 45 minutes, which is significantly faster than kits from other suppliers.

The novel Fast Reaction Mix is based on proven QIAGEN multiplex PCR technology that has been further optimized to increase inhibitor tolerance. Assay performance tested in the presence of increasing concentrations of 6 types of inhibitor (humic acid, hematin, tannic acid, indigo carmine, calcium, and collagen) shows high sensitivity and superior inhibitor resistance. This enables the generation of reliable, high-quality results for example directly from blood or for samples on FTA paper.

The Investigator IDplex GO! Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.

Principle

The Investigator IDplex GO! Kit uses QIAGEN's fast-cycling PCR technology for the rapid, simultaneous amplification of the 13 CODIS (Combined DNA Index System) core loci, D2S1338, D19S433, and Amelogenin. Carefully designed primers prevent overlap of loci alleles, thereby minimizing the likelihood of misinterpretation.

Procedure

The 15 polymorphic STR markers (D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, CSF1PO, FGA, TH01, TPOX, and vWA), and the gender-specific Amelogenin are amplified simultaneously in a single PCR and labeled with one of the dyes listed in the table (see the table Investigator IDplex GO! Kit dyes and corresponding markers).

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software. See the table Technical specifications for more information.

Investigator IDplex GO! Kit dyes and corresponding markers

Dye	Marker
6-FAM	Amelogenin	TH01	D3S1358	vWA	D21S11
BTG	TPOX	D7S82	D19S433	D5S818	D2S1338
BTY	D16S539	CSF1PO	D13S317	FGA
BTR	D18S51	D8S1179

Technical specifications

	Investigator IDplex GO! Kit	Investigator IDplex Plus Kit
DNA input volume	1.2 mm FTA punch or 2 µl lysate	Up to 15 µl
Volume per reaction	25 µl	25 µl
Matrix	BT5	BT5
Fluorescence dyes	6-FAM, BTG, BTY, BTO	6-FAM, BTG, BTY, BTO
Genetic Analyzers	ABI PRISM 3100/3100 Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500 Genetic Analyzers	ABI PRISM 310, ABI PRISM 3100/3100 Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500 Genetic Analyzers

Applications

The Investigator IDplex GO! Kit is for rapid and reliable generation of DNA profiles from blood and buccal swabs, and it is highly suited for:

Criminal database and forensic reference samples
Paternity testing
Population genetics studies
Other human identity testing applications

Product Resources

Validation Reports (8)

Sort options Sort alphabetically
	Investigator Validation Guide	Show details
	IDplex GO! Kit Validation Report	Show details
	Validation Certificate Investigator IDplex GO! Kit	Show details
	Validation Certificate STAR Q Punch AS Instrument	Show details
	Validation Certificate STAR Q Swab AS Instrument	Show details
	QIAGEN Exclusion Database Service	View
	Validation Certificate Investigator STR GO! Buffers	Show details
	Investigator 24plex GO! Kit Validation Report	Show details

Template Files (5)

Sort options Sort alphabetically
	(EN) - Investigator Template Files for Genotyper software	Show details
	Template File Info Note	Show details
	Investigator Template Files for GeneMapper ID v6 Software - (EN)	Show details
	Investigator Template Files for GeneMapper ID-X v10 CODIS Software For further information on which version of the v10 template files is suitable for your needs, please refer to the Template File Info Note.	Show details
	Investigator Template Files for GeneMapper ID-X v10 Standard Software For further information on which version of the v10 template files is suitable for your needs, please refer to the Template File Info Note.	Show details

Quick-Start Protocols (1)

Sort options Sort alphabetically
	EN - Investigator IDplex GO! Kit Quick Start Protocol	Show details

Technical Information (3)

Sort options Sort alphabetically
	Investigator IDplex Plus and IDplex GO! Kits - Technical Information	Show details
	QIAGEN Forensic-Grade Quality Technical Information	Show details
	(EN) - Overview of the markers of the Investigator human identification PCR Kits	Show details

Brochures & Guides (3)

Sort options Sort alphabetically
	QIAGEN Paternity Testing Solutions (eBook)	View
	No More Backlogs (eBook)	View
	Advanced Workflow Solutions for Confidence in Your Evidence (eBook)	View

Scientific Posters (1)

Sort options Sort alphabetically
	(EN) - Development of the Investigator STR GO! Kits for direct amplification of reference samples	Show details

Additional Resources (3)

Sort options Sort alphabetically
	ISO 18385 Forensic DNA Grade Certificate Investigator IDplex GO! Kit	Show details
	QIAGEN Forensic DNA Grade Quality	View
	ISO 18385 Forensic DNA Grade Certificate Investigator STR GO! Buffers	Show details

Kit Handbooks (1)

Sort options Sort alphabetically
	(EN) - Investigator IDplex GO! Handbook	Show details

FAQs (10)

Sort options Sort alphabetically
	Can additional positive controls be used for the Investigator IDplex GO! Kit? FAQ ID -3094	View
	Can the Investigator IDplex GO! PCR protocol be run on different PCR endpoint cyclers? FAQ ID -3089	View
	Do I need to evaluate optimal PCR cycle numbers for the Investigator IDplex GO! Kit with my samples? FAQ ID -3091	View
	Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit? FAQ ID -3090	View
	Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit? FAQ ID -3088	View
	Can the allelic ladder of the Investigator IDplex Plus Kit be used for analysis of Investigator IDplex GO! Kit? FAQ ID -3093	View
	Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit? FAQ ID -3095	View
	What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit? FAQ ID -3096	View
	Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay? FAQ ID -3092	View
	Is the Fast Reaction Mix ready to use in the Investigator IDplex GO? FAQ ID -3087	View

Application Notes (1)

Sort options Sort alphabetically
	Investigator STR GO! Kits on the Hamilton easyPunch STARlet Workstation	Show details

User-Developed Protocols (1)

Sort options Sort alphabetically
	Direct PCR amplification of STR loci from Bode Buccal DNA Collectors - (EN)	Show details

Safety Data Sheets (5)

Sort options Sort alphabetically
	MSDS Investigator IDplex GO! Kit (200)
	MSDS Investigator IDplex GO! Kit (1000)
	MSDS Investigator STR GO! Lysis Buffer (200)
	MSDS Investigator STR GO! Punch Buffer (1000)
	MSDS Investigator STR GO! Punch Buffer (200)