PyroMark Q96 MD Accessories and Reagents

For optimal sample preparation and Pyrosequencing analysis using PyroMark Q96 MD

Features

  • Reliable quantification of genetic or epigenetic DNA modifications
  • Solutions for quick and easy sample handling for successful results
  • Dedicated products for each platform and each step in the workflow
  • High-quality optimized reagents enable reliable Pyrosequencing results

Products

PyroMark Q96 MD Accessories and Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
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PyroMark Control Oligo

Cat. No. / ID: 979203

For installation check of system
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PyroMark Gold Q96 Reagents (5 x 96)

Cat. No. / ID: 972804

For performing Pyrosequencing reactions on the PyroMark Q96 ID (5 x 96) and PyroMark Q96 MD (15 x 96)
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PyroMark Gold Q96 CDT Reagents (6 x 96)

Cat. No. / ID: 972824

For performing Pyrosequencing reactions on the PyroMark Q96 MD in combination with the capillary dispensing tips (CDT)
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PyroMark Q96 HS Dispensing Tip Holder

Cat. No. / ID: 9019075

Reusable holder for tips, nucleotide dispensing tips, and reagent dispensing tips, for use with PyroMark Q96 MD
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PyroMark Gold Q96 Reagents (50 x 96)

Cat. No. / ID: 972807

For performing Pyrosequencing reactions on the PyroMark Q96 MD
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PyroMark Q96 HS Sample Prep Thermoplate

Cat. No. / ID: 9019071

Holder for placement of PyroMark Q96 Plate on heating block during annealing step prior to PyroMark Q96 MD analysis
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PyroMark Q96 HS Capillary Tip Holder

Cat. No. / ID: 9019076

Reusable holder for tips, nucleotide dispensing tips, and reagent dispensing tips, for use with PyroMark Q96 MD
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PyroMark Q96 HS Tip Holder Box

Cat. No. / ID: 9019074

Box for dust-free and safe storage of tips in between runs. For use with PyroMark Q96 MD
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PyroMark Q96 Vacuum Workstation (110 V)

Cat. No. / ID: 9001528

Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template
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PyroMark Q96 Vacuum Prep Trough (5)

Cat. No. / ID: 979011

Reusable plastic troughs for use with all PyroMark Q96 Vacuum Workstations
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PyroMark Q96 Vacuum Workstation (220 V)

Cat. No. / ID: 9001529

Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template
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PyroMark Q96 Vacuum Workstation (100 V)

Cat. No. / ID: 9001740

Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template
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PyroMark Denaturation Sol. (500 ml)

Cat. No. / ID: 979007

For denaturation of double-stranded PCR product into single-stranded DNA template
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PyroMark OneStep RT-PCR Kit (200)

Cat. No. / ID: 978803

For 200 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water
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PyroMark CpG Assay 96 well (200)

Cat. No. / ID: 978756

PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; plate format); minimum order of 24 assays per plate
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PyroMark PCR Kit (200)

Cat. No. / ID: 978703

For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water.
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PyroMark OneStep RT-PCR Kit (50)

Cat. No. / ID: 978801

For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water
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PyroMark CpG Assay 96 well (50)

Cat. No. / ID: 978754

PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (50 reactions; plate format); minimum order of 24 assays per plate
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PyroMark Binding Buffer (200 ml)

Cat. No. / ID: 979006

For binding of biotinylated PCR product to Sepharose beads
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PyroMark PCR Kit (800)

Cat. No. / ID: 978705

For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water
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PyroMark Vacuum Prep Filter Probe (100)

Cat. No. / ID: 979010

Reusable filter probes for PyroMark Vacuum Workstation Q96 and Q24
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PyroMark Annealing Buffer

Cat. No. / ID: 979009

For annealing of sequencing primer to DNA template
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PyroMark Custom Assay (200)

Cat. No. / ID: 978776

User-designed PCR and sequencing primers for Pyrosequencing analysis (200 reactions; tube format)
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PyroMark Wash Buffer (conc., 200 ml)

Cat. No. / ID: 979008

For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA
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PyroMark CpG Assay (200)

Cat. No. / ID: 978746

PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)

Product Details

The PyroMark Q96 MD uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q96 MD Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q96 MD Accessories and Reagents are designed specifically for use with PyroMark Q96 MD instruments. 

Please note, the following products will be discontinued as of January 31, 2021. We recommend the alternative product, PyroMark 48. For more information, please contact QIAGEN Technical Services.

PyroMark Q96 HS Plate (100) (cat. no. 979101)
PyroMark Q96 HS Q96 Reagent Tip (4) (cat. no. 979102)
PyroMark Q96 HS Capillary Tip (8) (cat. no. 979104)

Applications

PyroMark Q96 MD Accessories and Reagents enable Pyrosequencing analysis with the PyroMark Q96 MD instrument for applications such as:

  • HRM
  • Methylation analysis
  • Mutation analysis
  • SNP analysis

Resources

Kit Handbooks (7)
For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems
For performing Pyrosequencing reactions on the PyroMark Q96 ID, PyroMark Q96 MD, and PyroMark Q96 MD Automated
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
Application Notes (2)
A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.

Publications

Genetic diagnostics of functional variants of the human dopamine D2 receptor gene.
Doehring A; Kirchhof A; Lötsch J;
Psychiatr Genet; 2009; 19 (5):259-68 2009 Oct PMID:19512960
Pyrosequencing enhancement for better detection limit and sequencing homopolymers.
Gong M; Foo SH; Lin L; Liu ET; Gharizadeh B; Goel S;
Biochem Biophys Res Commun; 2010; 401 (1):117-23 2010 Sep 15 PMID:20833128
Rapid molecular identification of pathogenic yeasts by pyrosequencing analysis of 35 nucleotides of internal transcribed spacer 2.
Borman AM; Linton CJ; Oliver D; Palmer MD; Szekely A; Johnson EM;
J Clin Microbiol; 2010; 48 (10):3648-53 2010 Aug 11 PMID:20702674
[Quantitative pyrosequencing of heterozygous single nucleotide polymorphisms for rapid diagnosis of Down's syndrome].
Liu XQ; Wu HP; Bu Y; Zou BJ; Chen ZY; Zhou GH;
Zhonghua Yi Xue Yi Chuan Xue Za Zhi; 2009; 26 (3):331-5 2009 Jun PMID:19504451
Association between single nucleotide polymorphisms of surfactant protein D and allergic rhinitis in Chinese patients.
Deng YQ; Tao ZZ; Kong YG; Xiao BK; Chen SM; Xu Y; Wang Y; He Q;
Tissue Antigens; 2009; 73 (6):546-52 2009 Jun PMID:19493231

FAQ

What is the meaning of the abbreviations NDT and CDT and what are the differences?

The PyroMark Q96 MD uses the NDT and CDT to dispense nucleotides, while the Q24 and Q96 ID use cartridges.

NDT means nucleotide dispensing tip. This is the original dispensing tip which is more sensitive to dust but dispenses lower nucleotide volumes and enables longer read length.

CDT means caplillary dispensing tip and is comparable to Q24 and Q96 ID cartridges. They dispense a higher volume but are easier in handling.

The NDT and CDT use two different tip holders which cannot be interchanged

 

 

 

FAQ ID -2841
Can I order the nucleotides from PyroMark Gold Reagents separately?
The nucleotides can only be ordered as part of the PyroMark Gold Reagents which also contain enzyme and substrate mix.
FAQ ID -2827
How many times can the CDTs, NDTs, and RDTs be used?
The PyroMark Q96 MD HS Capillary Tip and HS Reagent Tip  can be used up to 20 times and the PyroMark Q96 HS Nucleotide Tip up to 10 times.
FAQ ID -2865
Which PyroMark Gold Q96 Reagent should be used for which instrument and application?

PyroMark Gold Q96 Reagents:

  • PyroMark Gold Q96 SQA Reagents (1 x 96) should be used for performing SQA analyses on the PyroMark Q96 ID. It can also be used to supplement the PyroMark Gold Q96 Reagents (5 x 96) when long runs lead to a shortage of nucleotides.
  • PyroMark Gold Q96 Reagents (5 x 96) should be used for SNP and CpG analyses on the PyroMark Q96 ID and MD instruments. On the PyroMark Q96 MD, the kit contains enough for 15x96 plates.
  • PyroMark Gold Q96 CDT Reagents (6 x 96) should be used only with CDTs on the PyroMark Q96 MD instrument, for performing SNP and CpG anslyses. The nucleotides are pre-diluted for use with CDTs, so this is not to be used on ID instrument or with NDTs on the MD.
  • PyroMark Gold Q96 Reagents (50 x 96) should be used only with the PyroMark Q96 MDA (automated option) for SNP anslyses. Not for use with the PyroMark Q96 ID or non-automated MD.

See the PyroMark Gold Q96 Reagents Handbook for additional information.

FAQ ID -2836
Can PyroMark Gold reagents be vortexed?
Reconstiuted enzyme and substrate of PyroMark Gold Reagents, should not be vortexed since this could lead to conformational changes which affect the activity.
FAQ ID -2844