REPLI-g FFPE procedure.
REPLI-g FFPE procedure.
High-molecular-weight ligated DNA.
High-molecular-weight ligated DNA.
Reliable microsatellite analysis.
Reliable microsatellite analysis.
REPLI-g FFPE principle.
REPLI-g FFPE principle.
REPLI-g FFPE procedure. Formalin fixation and paraffin embedding cause DNA fragmentation. During the REPLI-g FFPE procedure, these DNA fragments are randomly ligated and then amplified using multiple displacement amplification. As indicated, DNA fragments (containing different loci [L1–L4]) are not assembled in the original order. However, the different loci are equally amplified and can be detected in downstream genotyping applications.
High-molecular-weight ligated DNA.

DNA from different FFPE carcinoma tissues after (1a–5a) and before (1b–5b) amplification using the REPLI-g FFPE Kit was run on an agarose gel. The REPLI-g FFPE ligation procedure results in the formation of high-molecular-weight DNA. (Data kindly provided by Hartmut Schmidt, Gerhard Domagk Institute for Pathology, Münster, Germany.)
Reliable microsatellite analysis.

DNA from a 3.5-year-old FFPE carcinoma tissue sample was analyzed at a single microsatellite locus prior to and after DNA amplification using the REPLI-g FFPE Kit. Identical results were obtained for the original and REPLI-g amplified genomic DNA. (Data kindly provided by Hartmut Schmidt, Gerhard Domagk Institute for Pathology, Münster, Germany.)
REPLI-g FFPE principle.

The REPLI-g FFPE procedure involves a novel DNA processing reaction that prepares and ligates fragmented DNA. This ligated DNA is then used in MDA-based whole genome amplification.