Optimized buffers and enzymes lyse samples, stabilize nucleic acids and enhance selective RNA adsorption to the QIAamp membrane. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. Alcohol is added and lysates loaded onto the QIAamp spin column or 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use RNA is then eluted in water or low-salt buffer.
For greater speed and convenience in RNA purification, samples can be processed by vacuum instead of centrifugation. QIAamp Mini spin columns are accommodated on the QIAvac 24 manifold using VacValves and VacConnectors, provided in the QIAamp Vac Accessory Set. VacValves should be used if sample flow rates differ significantly to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination.
Automated processing on the QIAcube Connect
A dedicated QIAamp Viral RNA QIAcube Kit enables automated processing on the QIAcube Connect. In addition, the QIAamp Viral RNA Mini Kit is automatable on the QIAcube Connect. The QIAamp Viral RNA Mini Accessory Set provides the additional buffers and reagents needed for automated, low-throughput sample prep using both the QIAcube Connect and QIAamp Viral RNA Mini Kit.
Processing in 96-well format
The QIAamp 96 Viral RNA Kit is automatable on various instruments.
We recommend the use of the Centrifuge 4-16S or Centrifuge 4-16KS for centrifuge processing. Alternative centrifuges with swing-out rotor, minimum 5788 x g capacity and the ability to accommodate plate assemblies with a height of minimum 75 mm or more may be used. Centrifuges with lower g-force (minimum 3486 x g) can be used with an alternative protocol.
Plasticware recommended for centrifugation processing: Plastic consumables need to be in SBS format and must be able to withstand the necessary g-forces. Deep-well plates need to have a capacity of 2 ml and elution plates need to have a capacity of ≥500 µl. Four deep-well plates and one elution plate are required for processing of 96 samples.