Each well in an RT2 Profiler PCR Array Modification measures the relative expression of a gene related to a pathway or disease state. Arrays are available in four formats: the 96-well plate, the 384-well plate, the 100-well disc and the 96 x 96 chip. Every format includes comprehensive, proprietary controls in addition to the laboratory-verified gene assays, including controls to monitor genomic DNA contamination (GDC), first strand synthesis (RTC) and real-time PCR efficiency (PPC). You can add up to 4 genes of interest to replace 4 controls from the plate.
The RT2 Profiler PCR Array system comprises a complete experimental workflow, from sample preparation through to data analysis and interpretation, starting with just 25 ng of RNA (or <1 ng of RNA with the RT2 PreAMP cDNA Synthesis Kit) and provides free data analysis software. Each cataloged RT2 Profiler PCR Array incorporates laboratory-verified assays for 84 pathway-focused genes, 5 housekeeping genes for normalization and controls that check for sample quality and reaction quality.