High yields of double-stranded DNA.
High yields of double-stranded DNA.
GeneRead DNA FFPE Kit outperforms a kit from another supplier.
GeneRead DNA FFPE Kit outperforms a kit from another supplier.
Dramatic reduction in artifactual C>T|G>A transitions.
Dramatic reduction in artifactual C>T|G>A transitions.
Retention of high-frequency C>T|G>A transitions.
Retention of high-frequency C>T|G>A transitions.
Deamination of cytosine leads to false adenine pairing.
Deamination of cytosine leads to false adenine pairing.
High yields of double-stranded DNA. Single 10 μm slices of FFPE tissue were used to compare the results of DNA purification using standard and GeneRead protocols for FFPE samples. The wide range of DNA yields seen is due to the variation in the starting material. This is common for DNA purification from FFPE samples.
GeneRead DNA FFPE Kit outperforms a kit from another supplier. Double-stranded DNA was extracted from single 10 μm slices of FFPE tissue using the GeneRead DNA FFPE Kit and a product from an alternative supplier. Yields were measured by Qubit Fluorometric Quantitation.The GeneRead DNA FFPE Kit delivered up to 4-fold higher yields for the samples tested. A wide range of DNA yields is seen due to variation in the starting material, which is common for DNA extractions from FFPE samples.
Dramatic reduction in artifactual C>T|G>A transitions. Analysis of liver cancer samples was carried out. The GeneRead DNA FFPE Kit removed over 90% of low-frequency, novel mutations that are most likely to be artifactual in nature.
Retention of high-frequency C>T|G>A transitions. Analysis of liver cancer samples was carried out. High-frequency C>T|G>A transitions, whether novel or present in dbSNP, were retained when the GeneRead DNA FFPE protocol was used.
Deamination of cytosine leads to false adenine pairing. In FFPE samples, cytosine may become deaminated, leading to the cytosine pairing with adenine as uracil. In sequencing reactions, this will manifest as a C>T|G>A transition.
(Figure adapted from Klug and Cummings, 1997)