Investigator IDplex Kits

For multiplex amplification of 13 CODIS core loci, plus D2S1338, D19S433 and Amelogenin, also directly from swabs and papers

Features

  • Simultaneous amplification of 16 loci incl. CODIS core
  • Highly suitable for forensic casework or reference samples
  • High-quality results due to improved signal-to-noise ratio
  • Straightforward protocols with ready-to-use Fast Reaction Mix 
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Investigator IDplex Plus Kit (100)

Cat. No. / ID: 381625

Primer mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, allelic ladder IDplex Plus, DNA size standard 550 (BTO), and nuclease-free water
SEK 18,035.00
KitAccessories
Investigator STR Kit
Matrix Standard
Investigator STR GO! Buffer
Product type
IDplex Plus
IDplex GO!
Reactions
100
400
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Investigator IDplex Kits are intended for molecular biology applications in forensic, human identity and paternity testing. These products are not intended for diagnosis, prevention, or treatment of a disease. These products are not available in all countries.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

Investigator IDplex Kits are multiplex STR assays for human identification covering the 13 CODIS (Combined DNA Index System) core loci, D2S1338, D19S433 and Amelogenin. 

The kits utilize our fast-cycling technology, enabling amplification in just 45–90 minutes, depending on the kit used. Highly sensitive with rapid and reliable results from trace DNA, the Investigator IDplex Plus Kit is suitable for all forensic applications and paternity testing. With rapid and reliable results from reference samples such as buccal swabs, as well as buccal cells or blood on papers, the Investigator IDplex GO! Kit is suitable for reference testing in forensic applications and paternity testing. The genetic analyzer is calibrated with BT5 Matrix. 

Investigator IDplex Kits meet ISO 18385 requirements. 

Performance

Investigator IDplex Kits utilize fast-cycling technology to provide reliable results in STR assays in just 45 minutes for reference samples with direct amplification or 90 minutes for any other sample. 

The Fast Reaction Mix is based on proven QIAGEN multiplex PCR technology that has been optimized to increase inhibitor tolerance. Assay performance tested in the presence of increasing concentrations of 6 types of inhibitor (humic acid, hematin, tannic acid, indigo carmine, calcium and collagen) shows high sensitivity and superior inhibitor resistance. This enables reliable, high-quality results from casework samples, but also directly from reference samples such as blood or epithelial cells on FTA paper. 

Investigator IDplex Kits were validated and evaluated using the GeneAmp 9700 thermal cycler and the Applied Biosystems 3500 Genetic Analyzer. 

The recommended amount of DNA under standard conditions for use with the Investigator IDplex Plus Kit is 0.5 ng. Internal validations demonstrated robust and balanced results with 0.2–2 ng DNA, and reliable results with <0.1 ng DNA.

Principle

Investigator IDplex Kits use QIAGEN's fast-cycling PCR technology for the rapid, simultaneous amplification of the 13 CODIS (Combined DNA Index System) core loci, D2S1338, D19S433 and Amelogenin. Carefully designed primers reduce overlap of loci alleles, thereby minimizing the likelihood of misinterpretation.

Procedure

The 15 polymorphic autosomal STR markers (D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, CSF1PO, FGA, TH01, TPOX and vWA), and the gender-specific Amelogenin are amplified simultaneously in a single PCR and labeled with one of the dyes listed in the table "Investigator IDplex Kit dyes and corresponding markers". 

Investigator IDplex Kit dyes and corresponding markers

Dye Marker        
6-FAM Amelogenin TH01 D3S1358 vWA D21S11
BTG TPOX D7S820 D19S433 D5S818 D2S1338
BTY D16S539 CSF1PO D13S317 FGA  
BTR D18S51 D8S1179      

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as GeneMapper ID-X. See the table "Technical specifications" for more information.

Applications

Investigator IDplex Kits are for rapid and reliable generation of DNA profiles from blood, buccal swabs and forensic stains or reference samples and are highly suited for: 

  • Criminal database, forensic reference or casework samples 
  • Paternity testing
  • Population genetics studies
  • Other human identity testing applications 

Technical specifications

Features Investigator IDplex GO! Kit Investigator IDplex Plus Kit
DNA input volume 1.2 mm FTA punch or 2 µl lysate Up to 15 µl
Volume per reaction 25 µl 25 µl
Matrix BT5 BT5
Fluorescence dyes 6-FAM, BTG, BTY, BTO 6-FAM, BTG, BTY, BTO
Genetic analyzers ABI PRISM 3100/3100 Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500 Genetic Analyzers ABI PRISM 3100/3100 Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500 Genetic Analyzers
Software for analysis Applied Biosystems GeneMapper ID or GeneMapper ID-X Applied Biosystems GeneMapper ID or GeneMapper ID-X

Resources

Brochures & Guides (3)
Advanced sample collection, automation and STR solutions for kinship testing
Technical Information (4)

Enjoy success at first try with high performance QIAGEN Multiplex STR amplification and instrumentation

The most comprehensive and versatile coverage of current international STR standards


Quality initiatives for human identity testing and forensics 
Template Files (4)
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.

FAQ

Is the Fast Reaction Mix of the Investigator IDplex Plus Kit ready to use?
Yes. The Fast Reaction Mix of the Investigator IDplex Plus Kit contains HotStarTaq Plus enzyme, Mg2+, and dNTPs in a pre-optimized concentration. Only Investigator IDplex Plus Primer Mix and template DNA need to be added. Nuclease-free Water should be added to complete the reaction volume.
FAQ ID -3008
What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit?

We recommend first adding master mix to the PCR plate and then adding the punch to avoid punches getting lost or mixed up due to electrostatic effects. Furthermore, we strongly recommend spinning down the PCR plate after setup to ensure all punches get fully submerged.

FAQ ID -3096
Can the Investigator IDplex GO! PCR protocol be run on different PCR endpoint cyclers?
Yes. The protocol is generic and has successfully been tested on various commonly used instruments.
FAQ ID -3089
Can the PCR protocol of the Investigator IDplex Plus Kit be run on different PCR endpoint cyclers?
Yes. The protocol of the Investigator IDplex Plus Kit is generic and has been successfully tested on various commonly used instruments.
FAQ ID -3009
What is the maximum DNA input volume that can be used in the Investigator IDplex Plus Kit?
15 µl input volume can be used with the Investigator IDplex Plus Kit.
FAQ ID -3014
Can additional positive controls be used for the Investigator IDplex GO! Kit?

Amplification of Control DNA 9948 is appropriate as a PCR performance check. However, if you have access to reference samples at constant quality (for example, swabs or FTA donated by lab personnel), these samples may be used as additional in process controls.

FAQ ID -3094
Which filter set should be used with Investigator IDplex Plus Kit?
If you are using an ABI PRISM 3100-Avant/3100 or 3130/3130xl Genetic Analyzer, please use Any5Dye, if available. If you are using the Applied Biosystems 3500/3500xL Genetic Analyzer, please use filter set AnyDye. Otherwise, filter set G5 should be used. If you are using an ABI PRISM 310 Genetic Analyzer, filter set G5 should be used.
FAQ ID -3017
Does the Investigator IDplex Plus assay contain an internal control?
No. Unlike some other QIAGEN STR products (e.g., the Investigator Argus Y-12 QS Kit) there is no QS quality sensor included in the Investigator IDplex Plus Kit.
FAQ ID -3011
Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit?
No. Up to 2 µl can be added on top of a 25 µl reaction.
FAQ ID -3088
Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit?
We do not recommend using more than one punch of 1.2 mm diameter for blood or buccal cells on FTA, or 2 µl of a crude buccal swab lysate. To adapt protocols to the type and quality of samples used in your laboratory, and to the CE instrumentation in use, adjust PCR cycle numbers and CE injection conditions.
FAQ ID -3090
Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit?
Yes. Use of indicator cards helps to ensure sample is taken from an appropriate area of the FTA card. This may significantly improve success rates compared to procedures using non-indicator cards.

 

FAQ ID -3095
What is the optimal sample DNA concentration for the Investigator IDplex Plus Kit?
The optimum sample DNA input range of the Investigator IDplex Plus Kit is between 200–500 pg. However, reliable results can be obtained with less than 100 pg. For higher amounts of input DNA, consider adjusting the PCR cycle number.
FAQ ID -3015
Can the allelic ladder of the Investigator IDplex Plus Kit be used for analysis of Investigator IDplex GO! Kit?

No. Amplification products of marker FGA have a slightly different size between IDplex Plus and IDplex GO! kits due to a change in the fluorescent dye linker used. Use of the IDplex Plus ladder will frequently cause off ladder calls for FGA alleles.

 

FAQ ID -3093
Is the Fast Reaction Mix ready to use in the Investigator IDplex GO?

Yes. The Fast Reaction Mix contains Taq enzyme, Mg2+, and dNTPs in a pre-optimized concentration. Only IDplex GO! Primer Mix and sample needs to be added.

FAQ ID -3087
Do I need to evaluate optimal PCR cycle numbers for the Investigator IDplex GO! Kit with my samples?

Yes. Sample materials used for direct amplification vary with regard to quality and quantity of DNA present, depending on sampling materials, procedures, and storage conditions. In order to obtain the highest possible success rates in routine use, it is essential to run a representative batch of samples for evaluation of optimal conditions. Furthermore, we recommend revisiting this aspect after a certain period of routine use to ensure the evaluated conditions are optimal and re-adjust if necessary.

FAQ ID -3091
Have primer sequences of the Investigator IDplex Plus Kit been changed compared to the former Investigator IDplex assay?
No, the primer site locations of the markers in the Investigator IDplex Plus Kit have not been changed. Only minor changes have been introduced for some markers, e.g., to improve A addition to the PCR products.
FAQ ID -3012
What is the correct matrix to use with Investigator IDplex Plus Kit?
Matrix BT5 should be used with Investigator IDplex Plus Kit.
FAQ ID -3016
What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?

The BT5 matrix standard is designed for use with our Investigator STR and Dipplex Assays. We do not disclose the containing dyes.

FAQ ID - 3364
Is it possible to extend the number of PCR cycles to increase sensitivity of the Investigator IDplex Plus Kit?
Yes. For example, cycle numbers can be increased to 32 or 34 in order to obtain higher signals for very diluted samples. However, please note that this promotes the amplification of nonspecific products or amplification from spurious background DNA.
FAQ ID -3010
What does the Investigator IDplex Plus Kit contain?
The Investigator IDplex Plus Kit contains Primer mix, Fast Reaction Mix, Control DNA allelic ladder IDplex Plus, DNA size standard 550 (BTO), and nuclease-free water.
FAQ ID -3013
Where can I find the template files for the Investigator IDplex Plus Kit?
The template files for the Investigator IDplex Plus Kit for various softwares and analyzers can be downloaded from the Resource tab on the product webpage.
FAQ ID -3019
Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay?

No, primer site locations of markers have not been changed. Two additional SNP primers have been added to allow balanced amplification of vWA and D16S539 alleles carrying known primer binding site mutations. The mutations covered were observed in concordance studies in samples of African origin at an elevated level. The labelled FGA forward primer has been slightly changed with regard to the linker of the fluorescent dye. The change does not affect PCR amplification, but causes a slight size shift during capillary electrophoresis. The allelic ladder therefore has been changed accordingly.

FAQ ID -3092
Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator IDplex Plus Kit?

For Any5Dye filter set with the Investigator IDplex Plus Kit, please use:

  • Condition Bound: Lower 1.0 – Upper 20
  • Sensitivity: 0.1
  • Quality Value: 0.80
FAQ ID -3018