For sensitive detection of His-tagged proteins with an RGS·His epitope
Monoclonal mouse Anti·His Antibodies, like the Anti·His Antibody, are used to detect recombinant protein carrying His tags. RGS·His is one of three variants, each of which detects a different His tag epitope. Like all QIAGEN mouse monoclonal antibodies, RGS·His is prepared under serum-free conditions, which guarantee the absence of viruses, mycoplasma, and contaminating immunoglobulins.
Many different proteins have been detected, via their His tags, using the QIAexpress Anti·His Antibodies (see table). For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of QIAexpress Anti·His Antibodies)
All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody.
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
QIAexpress Detection Systems, with Anti·His Antibodies, allow efficient detection of His-tagged proteins and are ideal for many applications, including:
fragment fix placeholder