For sensitive, direct detection of His-tagged proteins
High-sensitivity and high-speed detection of His-tagged proteins
No need for secondary antibody
Complete kit with optimized blocking reagent
The Penta·His HRP Conjugate Kit is for sensitive detection of recombinant proteins carrying His tags without the need for secondary antibodies. It consists of Anti·His antibodies conjugated to horseradish peroxidase (HRP). In detection procedures, the Anti·His antibody binds to a protein's His tag and the complex is localized using chemiluminescent or chromogenic substrates that react with HRP.
125 μl Penta·His HRP Conjugate, 5 g Blocking Reagent, 50 ml Blocking Reagent Buffer (10x concentrate)
Performance of Penta·His HRP Conjugate Kit
N-terminal, C-terminal, and internal 6xHis tags
Sensitivity in dot blots:*
Sensitivity in western blots:*
Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
Stabilized stock solution
* Detection using chemiluminescent substrate. Sensitivity may vary with different proteins.
The Penta·His HRP Conjugate Kit consists of the QIAexpress Anti·His Antibodies chemically coupled to the reporter enzyme horseradish peroxidase (HRP). It can be used for highly specific and sensitive, direct detection of any recombinant protein that has an accessible His tag. The Anti·His HRP Conjugate Kits eliminate the need for secondary antibodies, saving time in immunodetection procedures.
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
The Penta·His HRP Conjugate Kit can be used with chemiluminescent or chromogenic detection substrates in: