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Quantinova LNA PCR lncRNA Focus Panels

For accurate and reliable lncRNA quantification using LNA-enhanced, SYBR® Green-based PCR
  • Expert-designed panels targeting the highly relevant pathways or diseases
  • Simultaneous profiling of related lncRNAs based on the latest literature
  • Exceptional sensitivity and specificity using short, LNA-enhanced primers
  • Stability for room-temperature setup allowing automation
  • Robust and reproducible results in just 2 hours using the QuantiNova SYBR® Green PCR Kit

QuantiNova LNA PCR lncRNA Focus Panels enable quick, accurate and sensitive expression analysis of disease- or pathway-related lncRNAs using SYBR® Green-based qPCR. LNA enhancement enables us to design shorter primers with optimal Tm, allowing flexible positioning on the target for optimal sequence discrimination. Thorough design validation ensures top performance and robust detection. Optimized using QuantiNova chemistry, the simple workflow can be completed in just 2 hours and is robust enough to support room-temperature setup, automation and delayed run. We also offer Flexible Panels, which you can create from the assays in our Focus Panels, as well as Custom Panels with your choice of predesigned and/or custom assays.

Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!

During the early release offering, only the mouse focus panels will be available. The human focus panels will be released in April 2020.

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Cat No./ID: 249951
QuantiNova LNA PCR lncRNA Focus Panels
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QuantiNova LNA PCR lncRNA Focus Panels Ready-to-use plate containing research area- or pathway-focused assays for lncRNA targets in 96- or 384-well format for any cycler. Minimum order is 1 plate; price discounted when ordering 6 or more copies of the same plate: 6-11 plates 25% off, 12-23 plates 40% off, >24 plates 55% off.

Quantinova LNA PCR lncRNA Focus Panels are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

9
QuantiNova LNA PCR Assays deliver consistent performance across all thermal cycler instruments.
Ten-fold dilutions of input cDNA ranging from 10 ng to 1 pg were prepared and then quantified with QuantiNova LNA PCR Assays for genes CCND1 and SEPRINA1 on 10 different PCR cyclers (fast-cycling if possible): Thermo Fisher QuantStudio, Thermo Fisher ViiA 7, ABI 7500 Standard, ABI 7500 FAST, ABI StepOne, Bio-Rad CFX96, Bio-Rad CFX-384, QIAGEN Rotor-Gene Q, Roche LightCycler 480, and Stratagene/Agilent Mx3000p. Results showed consistent and reproducible assay sensitivity and assay efficiency was high on all cyclers tested despite variations in cycling conditions, reaction volume and instrument formats. Shown are example amplification curves for CCND1 from 3 tested cyclers.
9
QuantiNova LNA PCR Assays enable both high-expression and low-expression detection of mRNA.
The PCR performance of QuantiNova LNA PCR Assays was evaluated using a commercially available DNA template (gBlock). DNA templates for ATP6V0D2, BRCA2, CCND1, CDK6, and PPA1 were serially diluted from 1x109 to 10 copies and quantified with QuantiNova LNA PCR Assays using a Bio-Rad CFX96 or Bio-Rad CFX384 instrument. The QuantiNova LNA PCR Assays exhibited high linear range combined with outstanding sensitivity and near optimal efficiency. This high linearity along the entire input range is essential to prevent biases in calculations of relative expression.
9
QuantiNova LNA PCR Assays provide accurate, sensitive and linear quantification of targets over a wide dynamic range.
Ten-fold dilutions of input cDNA ranging from 100 ng to 1 pg were prepared and quantified with QuantiNova LNA PCR Assays for 7 different mRNA targets on a Bio-Rad CFX96 or Bio-Rad CFX384 instrument. The assays exhibited accurate results at each dilution point demonstrating an efficiency of the assays over the full dilution range, as demonstrated by the example amplification curve shown (IFI16, 10 ng to 1 pg).
Performance
Expert LNA enhancement for top qPCR performance

The assays in the QuantiNova LNA PCR lncRNA Focus Panels have been developed using stringent design criteria and lab-validated algorithms. Twenty years of LNA design experience has enabled us to optimize our sophisticated LNA design algorithm, which incorporates over 50 different parameters to guarantee the most optimal assay for successful target detection. Each primer set delivers the highest specificity and efficiency for the most reliable and accurate results. LNA enhancement enables Tm normalization across the panel, giving the primers higher binding affinity,  dramatically increasing assay sensitivity and specificity. The increased sensitivity ensures excellent amplification efficiencies down to 1 RNA molecule, making it easier for you to detect low-abundance targets such as lncRNAs from less starting material (see figure QuantiNova LNA PCR Assays provide accurate, sensitive and linear quantification of targets over a wide dynamic range and figure QuantiNova LNA PCR Assays enable both high-expression and low-expression detection of mRNA). Increased specificity from the clever placement of LNA gives you a high signal-to-noise ratio, allowing discrimination of sequences that differ by only a single nucleotide and eliminating non-specific amplification and primer-dimer formation.

The high binding affinity of LNA bases increases the flexibility of primer placement on the transcript, so we are able to use intelligent positioning to design assays for otherwise difficult-to-analyze targets. This gives you robust and reliable quantification, even for AU-rich targets, low-abundance transcripts, targets with high secondary structure and highly complex samples.

Principle
High-performance SYBR® Green-based detection

QuantiNova LNA PCR Focus Panels use SYBR® Green-based detection, which, when used with a highly specific PCR system, well-designed primers and optimized reaction conditions, offers several advantages over hydrolysis probe detection. First, it is possible to perform melting curve analysis immediately following the PCR, so you can confirm high specificity of the reaction and rule out formation of primer–dimers or non-specific amplicons. It also provides higher flexibility in the assay design, because you only need two primers, instead of two primers and a probe. Furthermore, while the primers ensure the specificity of the detection, the dye’s binding properties enable analysis of many different targets without having to synthesize target-specific, labeled probes, reducing your assay setup and running costs.

Most comprehensive and specific coverage

QuantiNova LNA PCR lncRNA Focus Panels include the assays needed for a thoroughly researched panel of relevant pathway- or disease-focused long noncoding RNAs. The predesigned assays have been designed using our proprietary algorithm to provide the most sensitive, accurate and effective analysis and are transcript specific, enabling discrimination of closely related transcripts.

Flexible layout and controls

QuantiNova LNA PCR lncRNA Focus Panels are available in 96-well plate, 384-well plate and 100-well disc formats and are used to monitor the expression of 84 lncRNAs related to a disease state or pathway, plus multiple reference genes. Each lncRNA Focus Panel includes control elements for the following:

  • Data normalization
  • Genomic DNA contamination detection
  • RNA sample quality
  • General PCR performance
Multiple plate discounts
Plate quantity ordered Discount (%)
1–5 0
6–11 25
12–23 40
24+ 55
 
Complimentary data analysis

The complimentary, web-based data analysis tool in the GeneGlobe Data Analysis Center includes a user-friendly wizard to guide you step-by-step through the normalization and analysis of your data and generates publication-ready results figures.

Procedure
Choose one- or two-step qRT-PCR

QuantiNova LNA PCR lncRNA Focus Panels have been optimized using the time-tested and high-performance QuantiNova chemistry. Best results are obtained when reverse transcription is performed with the QuantiNova Reverse Transcription Kit. The resulting cDNA is then quantified by qPCR using the master mixes of the QuantiNova SYBR® Green PCR Kit combined with the assays on your panel.

For one-step qRT-PCR, we recommend using the QuantiNova SYBR® Green RT-PCR Kit, which offers quick reaction setup without need for optimization of reaction conditions. Simply combine RNA template with the ready-to-use RT-PCR master mix and the assays on your panel and start the reaction.

Fast and easy workflow

The fast and easy workflow can be completed in just 2 hours with minimal hands-on time and can be automated to save even more time and labor. The simple protocol has been tested for compatibility on all major brand qPCR cyclers (see figure QuantiNova LNA PCR Assays deliver consistent performance across all thermal cycler instruments). Furthermore, the cDNA generated in the RT step can be used across the entire QuantiNova LNA PCR System, allowing seamless transition from assays to panels depending on your research needs, saving you time and sample.

What you need to get started with the QuantiNova LNA PCR system

Reverse transcription: QuantiNova Reverse Transcription Kit

qPCR mastermix: QuantiNova SYBR® Green PCR Kit

One-Step qRT-PCR (optional): QuantiNova SYBR® Green RT-PCR Kit

Assays or panels:

  • QuantiNova LNA PCR Assays
  • QuantiNova LNA PCR Reference Assays
  • QuantiNova LNA PCR Custom Assays
  • QuantiNova LNA PCR Focus Panels
  • QuantiNova LNA PCR lncRNA Focus Panels
  • QuantiNova LNA PCR Custom Panels
  • QuantiNova LNA PCR Flexible Panels
Applications

QuantiNova LNA PCR lncRNA Focus Panels are highly suited for applications including:

  • lncRNA expression analysis, profiling and quantification
  • Validation of RNA-seq gene expression data
  • Gene expression profiling
  • Signal and pathway analysis
  • Confirming lncRNA expression knockdown by LNA GapmeRs or siRNAs
  • Biomarker development, including screening, identification and validation of disease-associated biomarkers
  • Monitoring phenotypic changes related to gene expression

Product Resources

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