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QIAseq FastSelect –rRNA HMR Kits

For rapid rRNA and/or globin mRNA removal for RNA-seq library preparation from human, mouse, rat and other mammalian samples
  • Compatible with QIAGEN, Illumina, NEB and KAPA stranded RNA-seq library kits
  • Single reagent for human, mouse, rat and other mammalian species
  • High-performance rRNA and/or globin removal in just 14 minutes
  • Only one pipetting step – combine QIAseq FastSelect reagent with RNA and incubate
  • No extra cleanup steps or NGS library protocol changes

QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits use a novel method to remove highly abundant RNA that is of low scientific value from your RNA-seq libraries. Researchers using RNA-seq for whole transcriptome analysis can use QIAseq FastSelect –rRNA HMR to remove cytoplasmic and mitochondrial rRNA, while researchers starting with blood samples can use QIAseq FastSelect –rRNA/Globin to remove both rRNA and globin mRNA. For researchers who are enriching for mRNA from blood, and only want to remove globin mRNA, we recommend QIAseq FastSelect –Globin. QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits remove more unwanted RNA and are 30% faster than our previous kit versions. 

We recommend using QIAseq Stranded RNA Library Kits for robust strand-specific RNA-seq library preparation for high-quality and highly fragmented (FFPE) RNA. 

Interested in customization of a QIAseq FastSelect –rRNA HMR or QIAseq FastSelect –Globin Kit for your samples? Contact us

Want to try the QIAseq FastSelect –rRNA/Globin Kit for the first time? Request a trial kit to evaluate.

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Cat No./ID: 334376
QIAseq FastSelect –Globin Kit (24)
11,205.00 kr
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Includes 3 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 24 reactions from human, mouse and rat samples
Cat No./ID: 334377
QIAseq FastSelect –Globin Kit (96)
40,355.00 kr
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Includes 1 tube of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 96 reactions from human, mouse and rat samples

Cat No./ID: 334378
QIAseq FastSelect –Globin Kit (384)
134,540.00 kr
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Includes 4 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 384 reactions from human, mouse and rat samples

Cat No./ID: 334386
QIAseq FastSelect –rRNA HMR Kit (24)
11,205.00 kr
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Includes 3 tubes of QIAseq FastSelect reagent for rRNA removal; sufficient for 24 reactions from human, mouse and rat samples

Cat No./ID: 334387
QIAseq FastSelect –rRNA HMR Kit (96)
40,355.00 kr
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Includes 1 tube of QIAseq FastSelect reagent for rRNA removal; sufficient for 96 reactions from human, mouse and rat samples

Cat No./ID: 334388
QIAseq FastSelect –rRNA HMR Kit (384)
134,540.00 kr
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Includes 4 tubes of QIAseq FastSelect reagent for rRNA removal; sufficient for 384 reactions from human, mouse and rat samples

Cat No./ID: 335376
QIAseq FastSelect –rRNA/Globin Kit (24)
17,840.00 kr
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Includes 3 tubes of QIAseq FastSelect reagent for rRNA removal and 3 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 24 reactions from human, mouse and rat samples

Cat No./ID: 335377
QIAseq FastSelect –rRNA/Globin Kit (96)
67,265.00 kr
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Includes 1 tube of QIAseq FastSelect reagent for rRNA removal and 1 tube of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 96 reactions from human, mouse and rat samples

Cat No./ID: 335378
QIAseq FastSelect –rRNA/Globin Kit (384)
224,240.00 kr
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Includes 4 tubes of QIAseq FastSelect reagent for rRNA removal and 4 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 384 reactions from human, mouse and rat samples

QIAseq FastSelect –rRNA HMR Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Performance

QIAseq FastSelect –rRNA HMR Kits demonstrated highly efficient removal of rRNA and superior performance compared to our previous kit versions and alternative products.
See figures:
QIAseq FastSelect –rRNA HMR Kit removes rRNA with 1 step in 14 minutes
QIAseq FastSelect –rRNA HMR Kit has comprehensive coverage to remove cytoplasmic and mitochondrial rRNA targets
QIAseq FastSelect –Globin Kit has comprehensive coverage to remove adult, fetal and embryonic globin mRNA RNA
QIAseq FastSelect –rRNA HMR Kit provides greater rRNA removal than previous kit versions
QIAseq FastSelect –rRNA HMR Kit shows comparable gene expression results with previous kit versions
QIAseq FastSelect –rRNA HMR Kit maintains the expression profile: detection of expressed genes (RPKM >0.3)
QIAseq FastSelect–rRNA HMR Kit maintains the expression profile: consistent detection of expressed genes (RPKM >0.3)

Robust performance with a variety of sample types, regardless of RNA input

QIAseq FastSelect provides reliable rRNA removal, consistent read mapping and reproducible gene expression results with RNA extracted from various sample types, including FFPE, and over a broad range of RNA input amounts.
See figures:
QIAseq FastSelect –rRNA HMR Kit is a single solution that removes mitochondrial and cytoplasmic rRNA from human, mouse, rat and other mammalian samples
QIAseq FastSelect –rRNA HMR Kit provides consistent distribution of read mapping between replicates
QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly efficient and reproducible removal of rRNA and globin mRNA, regardless of RNA input
QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly consistent breakdown of mapped reads, regardless of RNA input
QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: gene expression results
QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples
QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: removal of rRNA leads to increased exon mapping
QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: gene expression results
QIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples
QIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples: gene expression results

Principle

Most RNA removal or depletion strategies associated with RNA-seq library construction are sample pre-treatment strategies involving hybrid-capture or enzymatic removal of unwanted RNA. Our unique QIAseq FastSelect procedure is compatible with QIAGEN, Illumina, KAPA and NEB stranded library preparation kits and provides complete rRNA removal in a single, 14-minute inline step. This is dramatically faster than alternative RNA depletion kits, which require pre-treatment protocols involving more than 25 steps and 2 hours to complete. 

Simply add QIAseq FastSelect reagent (rRNA Removal and/or Globin Removal) to the RNA sample, perform fragmentation (if required), stepwise cool the reaction from 75°C to 25°C for 14 minutes and then complete the remaining library preparation steps. QIAseq FastSelect works with or without RNA fragmentation, providing the flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA as part of a standard RNA-seq library construction workflow.

Procedure

Most RNA removal or depletion strategies associated with RNA-seq library construction are sample pre-treatment strategies involving hybrid-capture or enzymatic removal of unwanted RNA. Our unique QIAseq FastSelect procedure is compatible with QIAGEN, Illumina, KAPA and NEB stranded library preparation kits and provides complete rRNA removal in a single, 14-minute inline step (figure QIAseq FastSelect Kit workflow). This is dramatically faster than alternative RNA depletion kits, which require pre-treatment protocols involving more than 25 steps and 2 hours to complete. 

Simply add QIAseq FastSelect reagent (rRNA Removal and/or Globin Removal) to the RNA sample, perform fragmentation (if required), stepwise cool the reaction from 75°C to 25°C for 14 minutes and then complete the remaining library preparation steps. QIAseq FastSelect works with or without RNA fragmentation, providing the flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA as part of a standard RNA-seq library construction workflow.

Applications

QIAseq FastSelect delivers rapid, reliable RNA removal from FFPE and fresh sample RNA sources. QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits are available in a variety of different formats and sizes to suit your specific applications. 

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