PyroMark Q24 Accessories and Reagents

For optimal sample preparation and Pyrosequencing analysis using the PyroMark Q24.

Features

  • Reliable quantification of genetic or epigenetic DNA modifications
  • Solutions for quick and easy sample handling for successful results
  • Dedicated products for each platform and each step in the workflow
  • High-quality optimized reagents enable reliable Pyrosequencing results

Products

PyroMark Q24 Accessories and Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
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PyroMark Q24 Validation Oligo

Cat. No. / ID: 979204

For performance confirmation of system
SEK 5,670.00
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PyroMark Gold Q24 Reagents (5 x 24)

Cat. No. / ID: 970802

Nucleotides, enzyme, and substrate solutions, intended for use with PyroMark Q24
SEK 2,275.00
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PyroMark Q24 Vacuum Workstation (220 V)

Cat. No. / ID: 9001518

Vacuum Workstation for preparing 24 samples in parallel from PCR product to single-stranded template
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PyroMark Denaturation Sol. (500 ml)

Cat. No. / ID: 979007

For denaturation of double-stranded PCR product into single-stranded DNA template
SEK 467.00
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PyroMark OneStep RT-PCR Kit (200)

Cat. No. / ID: 978803

For 200 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water
SEK 7,105.00
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PyroMark OneStep RT-PCR Kit (50)

Cat. No. / ID: 978801

For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water
SEK 2,400.00
Add to cart
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PyroMark PCR Kit (200)

Cat. No. / ID: 978703

For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water.
SEK 2,390.00
Add to cart
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PyroMark Q24 Cartridge (3)

Cat. No. / ID: 979202

Reusable dispensing tool for nucleotide and reagent delivery, for use with PyroMark Q24 and PyroMark Q24 Advanced
SEK 3,890.00
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PyroMark Binding Buffer (200 ml)

Cat. No. / ID: 979006

For binding of biotinylated PCR product to Sepharose beads
SEK 467.00
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PyroMark Q24 Plate (100)

Cat. No. / ID: 979201

24-well plate for samples, for use with PyroMark Q24 and PyroMark Q24 Advanced; 100 plates in each package
SEK 1,905.00
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PyroMark PCR Kit (800)

Cat. No. / ID: 978705

For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water
SEK 8,000.00
Add to cart
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PyroMark Vacuum Prep Filter Probe (100)

Cat. No. / ID: 979010

Reusable filter probes for PyroMark Vacuum Workstation Q96 and Q24
SEK 2,490.00
Add to cart
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PyroMark Control Oligo

Cat. No. / ID: 979203

For installation check of system
SEK 2,760.00
Add to cart
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PyroMark Q24 Vacuum Prep Troughs (12)

Cat. No. / ID: 979206

Reusable plastic troughs for use with all PyroMark Q24 Vacuum Workstations
SEK 330.00
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PyroMark Annealing Buffer

Cat. No. / ID: 979009

For annealing of sequencing primer to DNA template
SEK 467.00
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PyroMark Wash Buffer (conc., 200 ml)

Cat. No. / ID: 979008

For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA
SEK 467.00
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Product Details

The PyroMark Q24 uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q24 Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q24 Accessories and Reagents are designed specifically for use with the PyroMark Q24 instrument.

Resources

Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
Kit Handbooks (6)
For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems
For performing Pyrosequencing reactions on the PyroMark Q24
Application Notes (2)
A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.
Analysis Software (5)
BRAF Plug-in 1.3
SOFTWARE (1MB)
Version 1.3.0.7
For use with PyroMark Q24 Software version 2.0.8
GIST Plug-in 1.3
SOFTWARE (1MB)
Version 1.3.0.7
For use with PyroMark Q24 Software version 2.0.8
EGFR Plug-in 1.3
SOFTWARE (2MB)
Version 1.3.0.7
For use with PyroMark Q24 Software version 2.0.8
KRAS Plug-in 1.3
SOFTWARE (1MB)
Version 1.3.0.6
For use with PyroMark Q24 Software version 2.0.8
RAS Extension Plug-in 1.3
SOFTWARE (1MB)
Version 1.3.0.7
For use with PyroMark Q24 Software version 2.0.8

Publications

Genetic diagnostics of functional variants of the human dopamine D2 receptor gene.
Doehring A; Kirchhof A; Lötsch J;
Psychiatr Genet; 2009; 19 (5):259-68 2009 Oct PMID:19512960
[Quantitative pyrosequencing of heterozygous single nucleotide polymorphisms for rapid diagnosis of Down's syndrome].
Liu XQ; Wu HP; Bu Y; Zou BJ; Chen ZY; Zhou GH;
Zhonghua Yi Xue Yi Chuan Xue Za Zhi; 2009; 26 (3):331-5 2009 Jun PMID:19504451
Association between single nucleotide polymorphisms of surfactant protein D and allergic rhinitis in Chinese patients.
Deng YQ; Tao ZZ; Kong YG; Xiao BK; Chen SM; Xu Y; Wang Y; He Q;
Tissue Antigens; 2009; 73 (6):546-52 2009 Jun PMID:19493231
Global and gene-specific promoter methylation changes are related to anti-B[a]PDE-DNA adduct levels and influence micronuclei levels in polycyclic aromatic hydrocarbon-exposed individuals.
Pavanello S; Bollati V; Pesatori AC; Kapka L; Bolognesi C; Bertazzi PA; Baccarelli A;
Int J Cancer; 2009; 125 (7):1692-7 2009 Oct 1 PMID:19521983
BCL2-938C>A and CALCA-1786T>C polymorphisms in aseptic loosened total hip arthroplasty.
Wedemeyer C; Kauther MD; Hanenkamp S; Nückel H; Bau M; Siffert W; Bachmann HS;
Eur J Med Res; 2009; 14 (6):250-5 2009 Jun 18 PMID:19541585

FAQ

Can I order the nucleotides from PyroMark Gold Reagents separately?
The nucleotides can only be ordered as part of the PyroMark Gold Reagents which also contain enzyme and substrate mix.
FAQ ID -2827
Can PyroMark Gold reagents be vortexed?
Reconstiuted enzyme and substrate of PyroMark Gold Reagents, should not be vortexed since this could lead to conformational changes which affect the activity.
FAQ ID -2844