The QIAprep& CRISPR Kit offers a streamlined workflow, which combines a liquid-based sample preparation step that can be completed in only 25 minutes with sensitive PCR and Sanger sequencing detection (see figure The QIAprep& CRISPR Kit workflow). First, the target region is amplified using the QIAprep& CRISPR Kit and CRISPR-Q Custom PCR Assays. Following purification of the PCR product, it is sequenced using the CRISPR-Q Sanger Primers and the sequence traces are analyzed using the CRISPR-Q Sanger Sequencing Analysis tool, which is available in GeneGlobe.
Primers for Sanger sequencing of genomic regions of interest
CRISPR-Q Sanger Primers can be easily designed and ordered using the intuitive custom builder tool, which is also available in GeneGlobe. This tool generates several target-specific primers based on the genomic location and the sequence of the guide RNA used for your particular CRISPR gene editing events.