PAXgene Tissue RNA/miRNA Kit
For purification of total RNA, including miRNA, from tissues fixed and stabilized using the PAXgene Tissue System
The PAXgene Tissue RNA/miRNA Kit is intended fro the purification of total RNA, including miRNA, from PAXgene Tissue-fixed (PF) and PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue samples. The kit is intended to be used as part of the PAXgene Tissue System, which also includes the PAXgene Tissue FIX Container and PAXgene Tissue STABILIZER Concentrate for the collection, fixation, stabilization and storage of tissue samples.
Total RNA isolated with the PAXgene Tissue RNA/miRNA Kit is highly pure. Genomic DNA contamination is minimized and the purified RNA is ready to be used in downstream applications with no detectable inhibition of PCR. The resulting eluate includes smaller RNA molecules, such as 5.8S rRNA, 5S rRNA, tRNA and miRNAs. Studies have shown that the miRNA purified from PFPE tissue samples is reliably quantified, with high concordance to flash-frozen samples (see High concordance of miRNA expression between total RNA isolated from PFPE and flash-frozen tissue).
The PAXgene Tissue RNA/miRNA Kit enables purification of total RNA from tissues fixed and stabilized using the PAXgene fixation and stabilization reagents, which preserve tissue morphology and biomolecule integrity by avoiding destructive crosslinking and degradation found in formalin-fixed tissues. The purified RNA and miRNA have no inhibitory chemical modifications and thus, can be used in sensitive downstream applications (see RNA purified without chemical modification from PFPE tissue using the PAXgene Tissue RNA/miRNA Kit).
PAXgene Tissue-fixed (PF) or PAXgeen Tissue-fixed, paraffin-embedded (PFPE) tissue samples are distrupted and homogenized in binding buffer. After centrifugation to remove cellular debris, optimal conditions are created for binding of RNA molecules to the silica membrane. Contaminants are then washed away and DNase I treatment removes any trace amounts of DNA. Total RNA, including miRNA, is then eluted in a low-salt elution buffer and denatured by heating (see The PAXgene Tissue RNA/miRNA Procedure).
Total RNA and miRNA purified with the PAXgene Tissue RNA/miRNA Kit is ready to be used in a range of downstream research applications, including:
fragment fix placeholder