QIAamp One-For-All Nucleic Acid Kit

For automated purification of viral DNA and RNA or genomic DNA from a variety of sample types

Features

  • Rapid isolation of high-quality, viral DNA and RNA or genomic DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors
  • Flexibility with multiple sample types in the same run
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QIAamp One-For-All Nucleic Acid Kit (12)

Cat. No. / ID: 965672

For use on the BioRobot MDx. Includes: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, Elution Microtubes CL, caps, S-Blocks, carrier RNA
SEK 52,065.00
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The QIAamp One-For-All Nucleic Acid Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The QIAamp One-For-All Nucleic Acid Kit provides automated purification of viral nucleic acids or genomic DNA on the BioRobot MDx or the BioRobot Universal System using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run. The QIAamp One-For-All Nucleic Acid Kit enables processing of different samples (e.g., viral RNA and DNA from serum, genomic DNA from blood) in the same run.

Performance

The QIAamp One-For-All Nucleic Acid Kit can be used for a wide range of sample types, including, for example, cells (see figure " Reliable purification of EBV DNA") and whole blood (see figure " Efficient purification of viral DNA").

Consistent, high yields

The kit provides consistent high yields of viral nucleic acids over a wide range of viral loads (see figure " Reliable purification of viral DNA"). Automation ensures a high degree of standardization for consistent, repeatable results (see figure " Repeatable purification of viral nucleic acids").  DNA and RNA purified using the QIAamp One-For-All Nucleic Acid Kit can be used in a range of downstream applications, including:

  • PCR and quantitative real-time PCR and RT-PCR
  • Infectious disease research
See figures

Principle

The QIAamp One-For-All Nucleic Acid Kit simplifies isolation of viral RNA and DNA from a wide range of sample types with a fast vacuum procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in three efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit.

QIAamp sample preparation technology is fully licensed.

Procedure

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities. Viral nucleic acids, genomic DNA, and cellular RNA are eluted, and ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.

Applications

The QIAamp One-For-All Nucleic Acid Kit is designed to allow automated processing of multiple sample types in the same run. Sample types include:

  • Whole blood
  • Plasma and serum
  • CSF
  • Swabs
  • Urine

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, qPCR, real-time PCR
Samples per run (throughput)32-96 samples per run
For automated processingBioRobot MDx workstation or BioRobot Universal platform
Main sample typeCell-free body fluids like plasma, serum, CSF; whole blood; swabs; urine
TechnologySilica technology
Format96-well plates
Elution volume75 µl
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA and RNA, genomic DNA
ProcessingAutomated
Sample amount265 µl
Yield>90% recovery
Time per run or per prep<2.5 hours

Resources

Kit Handbooks (1)
For automated purification of nucleic acids from a wide range of sample types using the BioRobot MDx or BioRobot Universal System
Brochures & Guides (1)

FAQ

What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728