For simultaneous purification of viral DNA and RNA from plasma, serum, and cell-free body fluids
Rapid purification of high-quality viral DNA and RNA
No organic extraction or alcohol precipitation
Consistent, high yields
Complete removal of contaminants and inhibitors
The QIAamp MinElute Virus Spin Kit simplifies purification of viral DNA and RNA with fast spin-column procedures. The QIAamp MinElute Virus Spin Kit uses starting sample volumes of up to 0.2 ml and combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl. The QIAamp MinElute Virus Spin process can be fully automated on theQIAcube Connect.
The percentage of positive samples at low virus titers is shown. Nucleic acids from an RNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subject to RT-PCR. For the spin procedure, 95% probit values for 32 samples were 22.84 IU/ml and for the vacuum procedure, they were 9.46 IU/ml.
Viral nucleic acids purified using the QIAamp MinElute Virus Spin Kit can be used in a wide range of downstream applications, including:
PCR and quantitative real-time PCR
Infectious disease research
The QIAamp MinElute Virus Spin Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast spin-column procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids ready to use in PCR and blotting procedures.
The QIAamp MinElute Spin Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The procedure is suitable for use with plasma, serum, and other cell-free body fluids. QIAamp sample preparation technology is fully licensed.
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp MinElute membrane (see flowchart "QIAamp MinElute Virus Spin procedure"). Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.
The QIAamp MinElute Virus Spin Kit uses well-established technology for simultaneous purification of viral RNA and DNA from:
Fresh or frozen plasma
Other cell-free body fluids
PCR, real-time PCR
Main sample type
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
For 100 x 20 µl reactions: 1 x 500 µl QuantiNova Pathogen Master Mix, 1 x 500 µl Yellow Template Dilution Buffer, 1 x 250 µl ROX Reference Dye, 1 x 1.9 µl RNase-Free Water, 1 x 1500 µl Nucleic Acid Dilution Buffer, 1 x 100 µl Internal Control RNA, 1 x 100 µl Internal Control DNA, 1 x 200 µl IC Probe Assay