DNA was isolated from 50 mg of plant material (4 replicates each) using the DNeasy Plant Pro, DNeasy Plant Mini and DNeasy PowerPlant Pro Kits. DNA yields were quantified with a Qubit Fluorometer. (A) Plants with high DNA content (grass and pine needles); (B) Plants with lower DNA content (old strawberry, mint or citrus (lemon) leaf).
DNA from various plant samples was isolated with the DNeasy Plant Pro, DNeasy Plant Mini and DNeasy PowerPlant Pro Kits. An 8 µl aliquot of the resulting plant DNA eluates (3 replicates) was spiked into an internal control (IC) PCR and the IC was amplified using the QuantiFast Pathogen +IC Kit.
DNA was isolated from 50 mg samples of strawberry leaves. DNA yield was measured using a Qubit Fluorometer (A) and a 5 µl aliquot was analyzed by gel electrophoresis (B). An 8 µl aliquot of the resulting strawberry DNA eluates (4 replicates) was spiked into an internal control (IC) PCR (C ) and the IC was amplified using the QuantiFast Pathogen +IC Kit.
DNA was extracted from 50 mg plant material infected with the respective pathogen using the DNeasy Plant Pro Kit. A whole genome library was prepared with the QIAseq FX DNA Library Kit and sequenced using an Illumina MiSeq system (2 x 250 bp run). The resulting reads were analyzed with the CLC Genomic Workbench (QIAGEN Microbial Genomics Pro Suite).
