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REPLI-g UltraFast Mini Kit

For ultrafast highly uniform whole genome amplification from small or precious samples
  • Fast 1–1.5 hour whole genome amplification
  • Obtain unlimited DNA for downstream applications
  • Reproducible amplification from a variety of starting materials
  • Highly stable DNA suitable for long-term storage
A lack of sufficient quantities of genomic DNA for genomic analysis can be overcome by global amplification of all DNA within a sample (whole genome amplification). The REPLI-g UltraFast Kit includes REPLI-g UltraFast DNA Polymerase, which enables highly uniform and accurate whole genome amplification from small samples in just 60–90 minutes, resulting in typical DNA yields of 7 μg per 20 μl reaction. A variety of starting materials can be used, including genomic DNA, fresh or dried blood, buccal swabs, fresh or frozen tissue, and cells. The DNA generated is suitable for direct use in a range of genetic analyses.

Specifications

Features
Specifications
Amplification Whole genomic DNA
Applications Genotyping, microarray, PCR, real-time PCR
Denaturation step Alkaline
Maximum input volume 10 ng DNA, 0.5 µl whole blood, ~300 cells/µl
Minimal pipetting volume needed 1 µl
Quality assessment No
Reaction time 60–90 minutes
Reaction volume 20 µl
Samples per run (throughput) Mid
Starting amount of DNA >10 ng purified genomic DNA
Starting material Genomic DNA, blood, cells
Technology Multiple Displacement Amplification (MDA)
Yield ~10 µg

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Kit Handbooks (1)
For fast whole genome amplification from purified genomic DNA, blood, and cells
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