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REPLI-g Mini Kit

For highly uniform whole genome amplification from small or precious samples
  • Easy amplification with consistent yields of up to 10 µg
  • Unbiased amplification of genomic loci
  • Reliable results due to Multiple Displacement Amplification (MDA)
  • Amplified DNA highly suitable for most downstream applications
  • No risk of DNA degradation during long-term storage

The REPLI-g Mini Kit provides optimized reagents for whole genome amplification (WGA) from small samples using innovative Multiple Displacement Amplification (MDA) technology. The typical DNA yield of a 50 μl reaction is  up to 10 μg, with an average product length greater than 10 kb (ranging between 2 kb and 100 kb). Unique REPLI-g technology delivers highly uniform WGA from a variety of small or precious sample types, including purified genomic DNA, or directly from fresh or dried blood, buccal swabs, fresh or frozen tissue, and cells. This simple and reliable method is capable of accurate and unbiased amplification of genomes and generates DNA that can be applied without further purification or quantification for downstream applications that do not require labeling. In contrast to PCR-based WGA technologies, high fidelity rates are increased up to 1000-fold, avoiding costly false positive or negative results.

Specifications

Features
Specifications
Amplification Whole genomic DNA
Applications Genotyping, hybridization, RFLP
Denaturation step Alkaline
Maximum input volume >10 ng DNA, 0.1– 0.5 µl whole blood, >600 cells/µl
Minimal pipetting volume needed 0.5 µl
Quality assessment No
Reaction time 8–16 hours (overnight)
Reaction volume 50 µl
Samples per run (throughput) Mid
Starting amount of DNA >10 ng purified genomic DNA
Starting material Genomic DNA, blood, cells, tissue
Technology Multiple Displacement Amplification (MDA)
Yield 10–40 µg

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Kit Handbooks (1)
For whole genome amplification from purified genomic DNA, blood, and cells
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