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Gentra Puregene Yeast/Bact. Kit

For purification of archive-quality DNA from yeast and Gram-positive or Gram-negative bacteria
  • Archive-quality DNA for long-term storage
  • Reproducible DNA purification
  • A complete solution for sample-to-storage purification
  • Convenient, scalable purification procedure

The Gentra Puregene Yeast/Bact. Kit enables purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.

Specifications

Features
Specifications
Applications PCR, restriction digest, Southern analysis, SNP analysis
Elution volume 50 µl (varies)
Format Scalable
Main sample type Gram+ and Gram- Bacteria, Yeast
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Bacterial DNA / Yeast DNA
Sample amount 0.5-1.5*10e9 cells / 1-2*10e8 cells
Technology Modified salting-out precipitation method
Time per run or per prep 25 - 60 minutes (+ DNA rehydration)
Yield 17-50 µg/ml culture / 4.5 µg/ml culture

Product Resources

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Kit Handbooks (1)
For purification of archive-quality DNA from human whole blood, bone marrow, buffy coat, buccal cells, body fluids, cultured cells, tissue, mouse tail, yeast, bacteria
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Instrument Technical Documents (1)
Supplementary Protocols (4)
This protocol is designed for purification of DNA from 300 μl samples of whole blood using the Gentra Puregene Yeast/Bact. Kit.
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This protocol is designed for purification of DNA from 1 g samples of soil using the Gentra Puregene Yeast/Bact. Kit.
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This protocol is designed for purification of DNA from 0.5 or 1 ml samples of Gram-positive bacteria culture medium using the Gentra Puregene Yeast/Bact. Kit.
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This protocol is designed for purification of DNA from 5 ml samples of Gram-positive bacteria culture medium using the Gentra Puregene Yeast/Bact. Kit.
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