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EZ1 DNA Investigator Kit

For automated purification of DNA from 1–6 or 1–14 forensic and human identity samples per run
  • More efficient yields from trace casework samples
  • Higher signal-to-noise ratios
  • “Tip dance” protocol for processing solid material
  • Optional TE elution and use of carrier RNA

The EZ1 DNA Investigator Kit reproducibly automates purification of genomic DNA from reference and casework samples in human identity testing. The kit can be used with the EZ1 Advanced XL (1–14 samples per run), EZ1 Advanced (1–6 samples per run), and BioRobot EZ1 (1–6 samples per run) instruments. Purification is fast and efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios.

Specifications

Features
Specifications
Applications PCR, real-time RT-PCR, genotyping
Elution volume 50–200 µl
For automated processing BioRobot EZ1
Format Sample tube
Main sample type Forensic samples
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount <500 µl
Technology Magnetic particles
Yield 150–250 ng

Product Resources

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User-Developed Protocols (5)
This protocol has been adapted by the San Diego Police Department (SDPD) Crime Laboratory from the standard QIAGEN pretreatment for casework samples, and is intended for the lysis and extraction of DNA from forensic samples of fired (casings) and unfired (cartridges) ammunition.
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This protocol has been adapted by the North Louisiana Criminalistics Laboratory from the pretreatment for hair and is for the lysis and extraction of DNA from forensic samples of hair in less time than the standard EZ1 DNA Investigator protocol pretreatment.
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This protocol has been adapted by the North Louisiana Criminalistics Laboratory from the pretreatment for epithelial cells mixed with sperm cells and is for the lysis and extraction of DNA from a sperm pellet after differential separation.


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This protocol is adapted from the QIAGEN RNeasy MinElute Cleanup protocol and is intended for use as a guideline for the purification of total RNA from waste fractions obtained from a sample first purified using the EZ1 DNA Investigator protocol.
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Kit Handbooks (1)
For automated purification of DNA from forensic and biosecurity samples using EZ1 instruments
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Supplementary Protocols (4)
Supplementary protocol using the EZ1 Advanced DNA Investigator Large-Scale Bone Card or the EZ1 Advanced XL DNA Investigator Large-Scale Bone Card
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Supplementary protocol using the Flip Cap Rack and the EZ1 Advanced XL Flip Cap Card.
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Supplementary protocol using the Investigator Lyse&Spin Basket Kit and EZ1 Advanced XL
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Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
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