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QIAquick PCR Purification Kit

For purification of up to 10 μg PCR products, 100 bp to 10 kb
  • Up to 95% recovery of ready-to-use DNA
  • Cleanup of DNA up to 10 kb in three easy steps
  • Gel loading dye for convenient sample analysis

The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect.

For optimal results it is recommended to use this product together with QIAvac 24 Plus.

Specifications

Features
Specifications
Binding capacity 10 µg
Elution volume > 30 µl
Format Tube
Fragment size 100 bp – 10 kb
Fragments removed < 40mers
Processing Manual
Sample type: applications ssDNA or dsDNA from PCR and other enzymatic reactions
Technology Silica technology
Type(s) of DNA recovered ss DNA and dsDNA

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FAQs (19)
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User-Developed Protocols (1)
The protocol has been used successfully for Cy3-, Cy5-, and biotin-labeling of cDNA from <50 ng of total RNA or poly A+ mRNA.
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