text.skipToContent text.skipToNavigation

artus HI Virus-1 RT-PCR Kits RUO

For quantitative detection of HIV-1 specific RNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 76.4 IU/ml
  • Specific detection of HIV-1 genotypes A to H
  • Accurate quantitation of viral load over a very broad range
The artus HI Virus-1 RG RT-PCR Kit and the artus HI Virus-1 QS-RGQ Kit are ready-to-use molecular detection kits for real-time RT-PCR on Rotor-Gene Q instruments. The kits provide all necessary reagents optimized for HIV-1 RNA detection and quantitation. The artus HI Virus-1 RG RT-PCR Kit uses manual sample preparation and assay setup techniques. The artus HI Virus-1 QS-RGQ Kit is part of the QIAsymphony RGQ, the complete automated system from sample to pathogen detection, and is not for use with manual assay setup.
Cat No./ID: 4513233
artus HI Virus-1 RG RT-PCR Kit (24) RUO
For 24 reactions on Rotor-Gene Q instruments: 2 Masters, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4513303
artus HI Virus-1 QS-RGQ Kit (24) RUO
For 24 reactions on the QIAsymphony RGQ: 2 Masters, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Performance

To ensure sensitivity, artus HI Virus-1 Kits have been optimized to detect low numbers of HIV-1 RNA. The analytical sensitivity of the artus HI Virus-1 QS-RGQ Kit is 76.4 IU/ml in consideration of the purification and assay setup using the QIAsymphony RGQ system (see figure "Sensitive detection of HIV-1 RNA"). 

For reliable specificity, testing of the artus HI Virus-1 Kits was carried out using various HIV-1 isolates, including all group M subtypes A–H, and related pathogens.  

Performance of artus HI Virus-1 Kits
Kit  artus HI Virus-1 RG RT-PCR Kitartus HI Virus-1 QS-RGQ Kit
Sample type  EDTA plasma EDTA plasma
Analytical sensitivity  66.9 IU/ml 76.4 IU/ml
 Linear range  120 to >1 x 108 IU/ml  250 to 108 IU/ml
 Specificity  HIV-1, including all group M subtypes A–H  HIV-1, including all group M subtypes A–H

Principle

The artus HI Virus-1 RG RT-PCR Kit and the artus HI Virus-1 QS-RGQ Kit are based on the amplification and simultaneous detection of a specific region of the HIV-1 genome using real-time RT-PCR. The kits provide high levels of specificity and sensitivity (see figure "Sensitive detection of HIV-1 RNA") over a broad linear range.

Each artus HI Virus-1 Kit provides 4 HIV-1 quantitation standards (see figure "Reliable quantitation of HIV-1 load"). Use of the standards enables accurate quantitation of viral load. In addition, the kits contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical HIV-1 PCR is not reduced.

Specifications of artus HI Virus-1 Kits
Kitartus HI Virus-1 RG RT-PCR Kit and artus HI Virus-1 QS-RGQ Kit
Sample type EDTA plasma
Amplicon 93 nt region of the 5' LTR

Procedure

artus HI Virus-1 RT-PCR Kits provide all necessary reagents optimized for reliable HIV-1 RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

Complete automated system from sample to HIV-1 detection

The QIAsymphony RGQ workflow solution for HIV-1 detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus HI Virus-1 QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete workflow (see figure "Integrated QIAsymphony RGQ system for HIV-1 detection").

Recommendations for manual viral RNA purification

For the artus HI Virus-1 RG RT-PCR Kit, we recommend using the QIAamp DSP Virus Kit.

Applications

The artus HI Virus-1 RG RT-PCR Kit enables rapid and sensitive detection and quantitation of HIV-1 RNA purified from plasma using the QIAamp DSP Virus Kit.

The artus HI Virus-1 QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete workflow from sample to HIV-1 RNA detection and quantitation.

fragment fix placeholder