Due to the unique proprietary TopTaq Stabilizer contained in the enzyme storage buffer, TopTaq DNA Polymerase is the first PCR kit that can be stored at 4°C. TopTaq Stabilizer binds to TopTaq DNA Polymerase, preventing polymerase inactivation. Template DNA and primers are also prevented from binding to the polymerase at low temperatures. During the initial denaturation step, the TopTaq Stabilizer freely dissociates from the polymerase without compromising polymerase activity. This added convenience allows immediate reaction setup without the use of ice — saving the time usually required for reagent thawing.
[A] TopTaq DNA Polymerase was used to amplify 14 different PCR products from mammalian genomic DNA ranging in size from 100 bp to 2 kb according to the standard preoptimized protocol and using identical cycling conditions. [B] The same four targets (shown in lanes 11–14) were amplified under identical cycling conditions using DNA polymerases from the indicated suppliers. TopTaq DNA Polymerase (QIAGEN) resulted in high yields of specific PCR products, regardless of fragment size.
[A] TopTaq DNA Polymerase is stable at 4°C or room temperature due to the presence of low-molecular-weight molecules (TopTaq Stabilizer) on the surface of the polymerase. [B] At higher temperatures, the TopTaq Stabilizer completely dissociates from the polymerase without compromising polymerase activity.