mericon Pathogen Detection Assays

For real-time PCR detection of pathogen DNA in food or animal feed

Highly sensitive and specific detection of a broad range of pathogens
Single, streamlined detection protocol for all targets
Dedicated mericon bacterial DNA isolation kits available
PCR run time of 73 minutes on the QIAGEN Rotor-Gene Q
Full license for PCR without additional costs

mericon Pathogen Detection Assays are a suite of kits for the target-specific detection of bacterial pathogen DNA in food, animal feed, and pharmaceutical products. All of the assays use the same real-time PCR-based protocol. They are part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for ingredient authentication and GMO DNA detection. mericon assays perform optimally on the Rotor-Gene Q, but have also been validated for block thermal cyclers.

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Product Details
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Cat No./ID: 290013

mericon Salmonella spp Kit (24)

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For 24 reactions: PCR Assay Salmonella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290015

mericon Salmonella spp Kit (96)

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For 96 reactions: PCR Assay Salmonella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290023

mericon L. monocytogenes Kit (24)

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For 24 reactions: PCR Assay L. monocytogenes, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290025

mericon L. monocytogenes Kit (96)

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For 96 reactions: PCR Assay L. monocytogenes, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290033

mericon Campylobacter spp Kit (24)

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For 24 reactions: PCR Assay Campylobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290035

mericon Campylobacter spp Kit (96)

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For 96 reactions: PCR Assay Campylobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290043

mericon Campylobacter triple Kit (24)

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For 24 reactions: PCR Assay Campylobacter triple, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290045

mericon Campylobacter triple Kit (96)

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For 96 reactions: PCR Assay Campylobacter triple, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290053

mericon VTEC stx 1/2 Kit (24)

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For 24 reactions: PCR Assay VTEC stx 1/2, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290055

mericon VTEC stx 1/2 Kit (96)

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For 96 reactions: PCR Assay VTEC stx 1/2, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290063

mericon Cronobacter spp Kit (24)

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For 24 reactions: PCR Assay Cronobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290065

mericon Cronobacter spp Kit (96)

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For 96 reactions: PCR Assay Cronobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290073

mericon S. aureus Kit (24)

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For 24 reactions: PCR Assay S. aureus, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290085

mericon Quant Legionella spp Kit

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For 96 reactions: PCR Assay Quant Legionella spp, Internal Control, Standard DNA, Quant Control DNA, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290095

mericon Quant L. pneumophila Kit (96)

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For 96 reactions: PCR Assay Quant L. pneumophila, Internal Control, Standard DNA, Quant Control DNA, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290103

mericon Shigella spp Kit (24)

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For 24 reactions: PCR Assay Shigella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290105

mericon Shigella spp Kit (96)

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For 96 reactions: PCR Assay Shigella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290113

mericon Y. enterocolitica Kit (24)

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For 24 reactions: PCR Assay Y. enterocolitica, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290115

mericon Y. enterocolitica Kit (96)

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For 96 reactions: PCR Assay Y. enterocolitica, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290123

mericon Listeria spp Kit (24)

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For 24 reactions: PCR Assay Listeria spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290125

mericon Listeria spp Kit (96)

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For 96 reactions: PCR Assay Listeria spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

Cat No./ID: 290233

mericon E. coli STEC O-Type Kit (24)

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For 24 reactions: PCR Assay E. coli O157 STEC O-Type, Internal Control, Multiplex PCR Master Mix, RNase-Free Water

Cat No./ID: 290403

mericon E. coli O157 Screen Plus Kit (24)

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For 24 reactions: PCR Assay E. coli O157 Screen Plus, Internal Control, Multiplex PCR Master Mix, RNase-Free Water

Cat No./ID: 290405

mericon E. coli O157 Screen Plus Kit (96)

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For 96 reactions: PCR Assay E. coli O157 Screen Plus, Internal Control, Multiplex PCR Master Mix, RNase-Free Water

mericon Pathogen Detection Assays are intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Performance

mericon Campylobacter spp Kit

The mericon Campylobacter spp Kit is for the specific detection of several Campylobacter subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Campylobacter DNA in a reaction and it exhibits high specificity for pathogens this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Campylobacter spp Kit cross-reactivity study").

mericon Campylobacter triple Kit

The mericon Campylobacter triple Kit is designed for the detection of Campylobacter jejuni, C. coli, and C. laridis in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of C. jejuni, C. coli, or C. laridis DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Campylobacter triple Kit cross-reactivity study").

mericon Cronobacter spp Kit

The mericon Cronobacter spp Kit is for the specific detection of Chronobacter subspecies in enrichment cultures of food and animal feed samples. Due to a recent change in taxonomy and according to regulation of the EU Commission (365/2010) the name Cronobacter spp replaces Enterobacter sakazakii. The assay can detect as few as 10 copies of Cronobacter DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Cronobacter spp Kit cross-reactivity study").

mericon Quant Legionella spp Kit

The mericon Quant Legionella spp Kit is for the specific detection and quantification of several Legionella species in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of Legionella DNA in a reaction and it exhibits high specificity for pathogens of that genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant Legionella spp Kit cross-reactivity study").

mericon Quant L. pneumophila Kit

mericon Listeria spp Kit

The mericon Listeria spp Kit is for the specific detection of several Listeria subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Listeria DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Listeria Kit cross-reactivity study").

mericon L. monocytogenes Kit

The mericon L. monocytogenes Kit is for the specific detection of Listeria monocytogenes in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of L. monocytogenes DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens, even closely-related Listeria species (see the table "Results of mericon L. monocytogenes Kit cross-reactivity study").

mericon Salmonella spp Kit

The mericon Salmonella spp Kit is for the specific detection of several Salmonella subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Salmonella DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Salmonella spp Kit cross-reactivity study").

mericon Shigella spp Kit

The mericon Shigella spp Kit is for the specific detection of several Shigella subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Shigella DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Shigella spp Kit cross-reactivity study").

mericon S. aureus Kit

The mericon S. aureus Kit is for the specific detection of Staphylococcus aureus in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of S. aureus DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon S. aureus Kit cross-reactivity study").

mericon VTEC stx1/2 Kit

The mericon VTEC stx1/2 Kit is for the specific detection of the strains of Escherichia coli that produce verotoxins, also called Shiga toxins, in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of verotoxin-producing E. coli DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon VTEC stx1/2 Kit cross-reactivity study").

mericon Y. enterocolitica Kit

The mericon Y. enterocolitica Kit is designed for the detection of Yersinia enterocolitica in enrichment cultures of food and animal feed samples. The kit specifically detects human pathogen serotypes 0:3, 0:5, 0:6, 0:8, 0:9, and 0:5:27. The assay can detect as few as 10 copies of Yersinia enterocolitica DNA in a reaction and exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Y. enterocolitica Kit cross-reactivity study").

Results of *mericon* Campylobacter spp Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Campylobacter jejuni	+	Campylobacter coli	+
Campylobacter laridis	+	Campylobacter upsaliensis	+
Salmonella spp.	–	VTEC stx1	–
Clostridium perfringens	–	VTEC stx2	–
Legionella erythra	–	Yersinia enterocolitica	–
Staphylococcus aureus	–	Listeria monocytogenes	–

Results of *mericon* Campylobacter triple Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Campylobacter jejuni	+	Campylobacter coli	+
Campylobacter laridis	+	Campylobacter upsaliensis	–
Salmonella spp.	–	VTEC stx1	–
Clostridium perfringens	–	VTEC stx2	–
Legionella pneumophila	–	Yersinia enterocolitica	–
Staphylococcus aureus	–	Listeria monocytogenes	–
Escherichia coli	–	Bacillus cereus	–

Results of *mericon* Cronobacter spp Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Cronobacter sakazakii	+	Listeria monocytogenes	–
Staphylococcus aureus	–	Escherichia coli	–
Salmonella spp.	–	Legionella erythra	–
Clostridium perfringens	–	Bacillus cereus	–
Legionella pneumophila	–	Yersinia enterocolitica	–
Shigella flexneri	–	VTEC stx1	–
Campylobacter jejuni	–	VTEC stx2	–

Results of *mericon* Legionella spp Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Legionella pneumophila	+	Legionella erythra	+
Salmonella thyphimurium	–	Escherichia coli	–
Shigella flexneri	–	Bacillus cereus	–
Clostridium perfringens	–	Yersinia enterocolitica	–
Campylobacter jejuni	–

Results of *mericon* L. pneumophila Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Legionella pneumophila	+	Legionella erythra	–
Salmonella thyphimurium	–	Escherichia coli	–
Shigella flexneri	–	Bacillus cereus	–
Clostridium perfringens	–	Yersinia enterocolitica	–
Campylobacter jejuni	–

Results of *mericon* Listeria spp Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Listeria monocytogenes	+	Cronobacter sakazakii	–
Listeria welshimeri	+	Legionella pneumophila	–
Listeria seeligeri	+	Staphylococcus aureus	–
Listeria ivanovii	+	Bacillus cereus	–
Listeria innocua	+	Yersinia enterocolitica	–
Listeria marthii	+	VTEC	–
Listeria grayi	+	Legionella erythra	–
Listeria rocoutiae	+	Shigella flexneri	–
Campylobacter jejuni	–	Clostridium perfringens	–
Escherichia coli	–

Results of *mericon* L. monocytogenes Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Listeria monocytogenes	+	Cronobacter sakazakii	–
Clostridium perfringens	–	Bacillus cereus	–
Staphylococcus aureus	–	Legionella erythra	–
Salmonella spp.	–	Campylobacter jejuni	–
Yersinia enterocolitica	–	Legionella pneumophila	–
Shigella flexneri	–	VTEC stx1	–
Escherichia coli	–	VTEC stx2	–
Listeria innocua	–	Listeria welshimeri	–
Listeria ivanovii spp. Ivanovii	–	Listeria grayi	–
Listeria seeligeri	–

Results of *mericon* Salmonella spp Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Salmonella spp	+	Cronobacter sakazakii	–
Clostridium perfringens	–	Bacillus cereus	–
Staphylococcus aureus	–	Legionella erythra	–
Listeria monocytogenes	–	Campylobacter jejuni	–
Yersinia enterocolitica	–	Legionella pneumophila	–
Shigella flexneri	–	VTEC stx1	–
Escherichia coli	–	VTEC stx2	–

Results of *mericon* Shigella spp Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Shigella spp.	+	Listeria monocytogenes	–
Staphylococcus aureus	–	Escherichia coli	–
Salmonella spp.	–	Bacillus cereus	–
Clostridium perfringens	–	Yersinia enterocolitica	–
Campylobacter jejuni	–	Legionella erythra	–
VTEC stx1	–	VTEC stx2	–

Results of *mericon* S. aureus Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Staphylococcus aureus	+	Listeria monocytogenes	–
Cronobacter sakazakii	–	Escherichia coli	–
Salmonella spp.	–	Legionella erythra	–
Clostridium perfringens	–	Bacillus cereus	–
Legionella pneumophila	–	Yersinia enterocolitica	–
Shigella flexneri	–	VTEC stx1	–
Campylobacter jejuni	–	VTEC stx2	–

Results of *mericon* VTEC stx1/2 Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
VTEC stx1	+	VTEC stx2	+
Campylobacter jejuni	–	Escherichia coli	–
Salmonella spp.	–	Legionella erythra	–
Clostridium perfringens	–	Bacillus cereus	–
Legionella pneumophila	–	Yersinia enterocolitica	–
Staphylococcus aureus	–	Listeria monocytogenes	–

Results of *mericon* Y. enterocolitica Kit cross-reactivity study
Pathogen	Result	Pathogen	Result
Yersinia enterocolitica	+	Listeria monocytogenes	–
non-pathogenic Yersinia enterocolitica	–	Escherichia coli	–
Staphylococcus aureus	–	Bacillus cereus	–
Clostridium perfringens	–	Salmonella spp.	–
Campylobacter jejuni	–	Legionella erythra	–
VTEC stx1	–	VTEC stx2	–

The mericon Quant L. pneumophila Kit is for the specific detection and quantification of Legionella pneumophila in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of L. pneumophila DNA in a reaction and it exhibits high specificity for L. pneumophila . In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogensThe mericon Quant Legionella spp Kit is for the specific detection and quantification of several Legionella species in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of Legionella DNA in a reaction and it exhibits high specificity for pathogens of that genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant Legionella spp Kit cross-reactivity study").

Principle

Detection of pathogens using real-time PCR is based on the amplification of a specific region of the relevant pathogen genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

mericon PCR Assays include an internal control to control potential inhibiton. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").

The PCR primer set for each assay is highly specific and targets a unique and conserved region of the tested pathogen genome that has been bioinformatically and experimentally verified.

Summary of possible PCR outcomes
Internal control amplified	Tested DNA amplified	Meaning
+	+	Sample is positive for the target DNA
+	–	Sample is negative for the target DNA
–	–	PCR failed due to inhibition

Procedure

DNA is extracted from an enrichment culture of a sample of food or animal feed using a dedicated sample preparation kit. The mericon Bacteria Kit is for the isolation of bacterial DNA from Gram-negative bacteria. The mericon Bacteria Plus Kit is for the isolation of bacterial DNA from Gram-positive bacteria.

The isolated, pure bacterial DNA is assayed using a streamlined real-time PCR protocol that returns results in 73 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.

The results are displayed as easy-to-interpret PCR curves.

Applications

mericon Pathogen Detection Assays are for real-time PCR-based detection of specific bacterial pathogens in food, animal feed, and pharmaceutical products.

Specifications

Features	Specifications
Quantitative/qualitative	Qualitative (Quantitative for Quant L pneumophilia and Quant Legionella spp)
Recommended sample prep	mericon DNA Bacteria Kit (Gram-negative bacteria) or mericon DNA Bacteria Plus Kit (Gram-positive bacteria) or QIAamp UCP Pathogen Mini Kit (Legionella-specific)
RUO/CE/ASR	RUO
Sample type	Bacterial preculture from food and water samples after concentration, e.g. filtration (for Legionella- specific Kits)
Thermal cycler	Rotor-Gene Q and other cyclers
What detected	DNA of respective pathogen

Product Resources

Brochures & Guides (5)

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	(EN) - Food Safety Testing Solutions by QIAGEN	Show details
	(EN) - Food pathogen screening with mericon solutions	Show details
	Food Pathogen Screening (eBook)	View
	(EN) - QIAGEN food safety testing technologies	Show details
	Application Guide — QIAxcel Advanced (eBook)	View

Scientific Posters (1)

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	(EN) - Novel solutions for food safety testing	Show details

Kit Handbooks (7)

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	(EN) - mericon Pathogen Detection Handbook	Show details
	(EN) - mericon Automated Pathogen Detection Workflow	Show details
	Product Insert for the AOAC-RI PTM-certified Automated and Manual mericon E. coli O157 Screen Plus and mericon® E. coli STEC O-type Detection Workflow	Show details
	mericon E. coli STEC O-Type Detection Handbook	Show details
	(EN) - Validated mericon Listeria Detection Workflow Product Insert	Show details
	(EN) - Product Insert for the AOAC-RI PTM-certified mericon Automated and Manual Salmonella Detection Workflows	Show details
	(EN) - mericon Quant Legionella Detection Handbook	Show details

Quick-Start Protocols (3)

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	(EN) - mericon Pathogen Detection Kits on the Rotor-Gene Q	Show details
	mericon E coli STEC O-Type Kit Quick Start Protocol	Show details
	(EN) - mericon Quant Legionella Detection on the Rotor-Gene Q	Show details

Template Files (1)

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	Rotor-Gene Q Template Files for mericon E coli STEC OType Rotor-Gene Q template files for starting and analyzing PCR runs	Show details

Application Notes (1)

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	Detection of salmonella in chocolate matrices using mericon assays and QIAsymphony RGQ - (EN)	Show details

Instrument Technical Documents (1)

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	Informational flyer on the mericon VTEC stx 1/2 assay - (EN)	Show details

Safety Data Sheets (15)

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	MSDS mericon Campylobacter spp Kit (96)
	MSDS mericon Campylobacter triple Kit (24)
	MSDS mericon Cronobacter spp Kit (96)
	MSDS mericon E. coli O157 Screen Plus Kit (96)
	MSDS mericon E. coli STEC O-Type Kit (24)
	MSDS mericon Listeria spp Kit (24)
	MSDS mericon L. monocytogenes Kit (96)
	MSDS mericon S. aureus Kit (24)
	MSDS mericon Salmonella spp Kit (24)
	MSDS mericon Salmonella spp Kit (96)
	MSDS mericon Shigella spp Kit (24)
	MSDS mericon VTEC stx 1/2 Kit (96)
	MSDS mericon Y. enterocolitica Kit (24)
	MSDS mericon Quant Legionella spp Kit
	MSDS mericon Quant L. pneumophila Kit (96)