Plasmid pCMVβ DNA was prepared using the indicated preparation method with standard and high-yield (HY) protocol for QIAGEN Plasmid Plus Kits. Huh-7 cells were transfected using 200 ng plasmid DNA and 0.5 μl Attractene Transfection Reagent or 300 ng plasmid DNA and 0.75 μl Attractene Transfection Reagent.
Purification per pellet-wet weight (g/l) for midi prep using Buffer ETR is shown. QIAGEN Plasmid Plus Technology generally results in low endotoxin levels. Buffer ETR technology further decreases these levels.
QIAGEN Plasmid Plus Kits result in lower EU content levels than kits based on silica and anion-exchange technology from other suppliers. For EndoFree Plasmid Kits, the EU content is below detection range.
Comparison of plasmid DNA yields using different methods — QIAGEN Plasmid Plus high-yield protocol (QPP), QIAGEN Plasmid Plus standard protocol (QPP), QIAGEN Plasmid Kit (QP), Supplier I, and Supplier S — is shown. Microgram DNA/ml biomass is indicated.
Plasmid DNA (100 μg) purified using the QIAGEN Plasmid Plus Mega Kit was analyzed using HPLC on an analytical anion-exchange column to detect non-plasmid nucleic acid contamination (i.e., RNA or genomic DNA). No contamination is present in the plasmid prep.