text.skipToContent text.skipToNavigation

PAXgene Blood DNA Kit

For purification of up to 500 µg genomic DNA from whole blood
  • Blood collection and purification in one system
  • Easy handling
  • Enhanced workflow efficiency

The PAXgene Blood DNA system consists of PAXgene Blood DNA Tubes for blood collection and stabilization, and the PAXgene Blood DNA Kit for DNA purification in a single-tube procedure.

Buy Products

Cat No./ID: 761133
PAXgene Blood DNA Kit (25)
Processing tubes and buffers for 25 preparations
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

Product Details

DNA isolated using the PAXgene Blood DNA System has an A260/A280 ratio of 1.7–1.9. Fragments are 20–200 kb in size, with an average length of 50–150 kb (see figure "High quality and high molecular weight of genomic DNA"). Average DNA yields are 150–500 µg, depending on the number of nucleated cells present in the blood sample. Purified DNA can be used in PCR and quantitative, real-time PCR (see figure "Efficient multiplex PCR of 3 mitochondrial genes").

The PAXgene Blood DNA System is an integrated and standardized system for collection and stabilization of whole blood specimens and isolation of their genomic DNA. The system requires the combined use of PAXgene Blood DNA Tubes for blood collection and stabilization, followed by DNA isolation using the PAXgene Blood DNA Kit.

Blood is collected into PAXgene Blood DNA Tubes, which contain a proprietary blend of reagents that both prevents blood coagulation and stabilizes white blood cells. Blood samples can be stored in the tubes for up to 14 days at room temperature. DNA is isolated from the tubes using appropriate buffers supplied in the PAXgene Blood DNA Kit. Highly pure genomic DNA is obtained, suitable for use in a wide range of downstream applications.


The PAXgene Blood DNA procedure is shown in the flowchart "PAXgene Blood DNA procedure". Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer.


The DNA can be used in a wide range of downstream applications, including:

  • PCR and quantitative, real-time PCR 
  • Southern blotting
  • SNP genotyping
  • Pharmacogenomic studies
  • SNP discovery and Validation


Applications PCR, qPCR, real-time PCR, blotting, genotyping
Elution volume 1 ml
Format Processing tube (salting out method)
Main sample type Whole blood
Processing Manual (centrifugation)
Sample amount 8.5 ml
Time per run 60 min/8 samples
Yield 150–500 µg

Product Resources

You are not authorized to download the resource

Brochures & Guides (3)
Second edition — innovative tools
Show details

Show details
For collection, storage, and transport of whole blood for genomic DNA isolation


Show details
FAQs (18)
FAQ ID -12
FAQ ID - 3497
FAQ ID - 3498
FAQ ID - 3499
FAQ ID - 3500
FAQ ID - 3501
FAQ ID - 3502
FAQ ID - 3503
FAQ ID - 3504
FAQ ID - 3505
FAQ ID - 3507
FAQ ID - 3508
FAQ ID - 3509

FAQ ID - 3511

FAQ ID - 3512

FAQ ID - 3515
FAQ ID - 3506
FAQ ID - 3510
Kit Handbooks (1)
For isolation of genomic DNA from 8.5 ml human whole blood
Show details
fragment fix placeholder